{"doi":"10.1006/cbir.1999.0458","title":"ARE CELLS RESCUED FROM ‘LOW DENSITY DEATH’ BY CO‐OPERATION BETWEEN PHOSPHOLIPASES C AND D?","abstract":"<jats:title>Abstract</jats:title><jats:p>Cells of the ciliate <jats:italic>Tetrahymena thermophila</jats:italic> die when transferred at low density to a lipid‐free nutritionally complete medium. This death is prevented and they will start to proliferate if protein kinase C is activated and this activation is sustained. We propose that this takes place in two stages. Firstly, the phospholipase C pathway beginning with and specific for phosphatidylinositol leads to the formation of diacylglycerol and inositol <jats:italic>tris</jats:italic> ‐phosphate. Diacylglycerol activates protein kinase C, and inositol <jats:italic>tris</jats:italic> ‐phosphate via Ca<jats:sup>2+</jats:sup>phospholipase D (PLD). Secondly, the protein kinase C response can now be sustained by diacylglycerol produced by phospholipase D, using phosphatidylcholine and phosphatidylserine as substrates. Should this switching from PI‐specific phospholipase C (PLC) to phospholipase D fail, then the cell will die in the course of milliseconds during the minutes following inoculation.</jats:p>","journal":"Cell Biology International","year":2000,"id":663448,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1732164,"name":"Leif Rasmussen","orcid":null,"position":1,"is_corresponding":false},{"id":271146,"name":"Morten Rasmussen","orcid":"0000-0001-7491-4137","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"ARE CELLS RESCUED FROM ‘LOW DENSITY DEATH’ BY CO‐OPERATION BETWEEN PHOSPHOLIPASES C AND D?","abstract":"<jats:title>Abstract</jats:title><jats:p>Cells of the ciliate <jats:italic>Tetrahymena thermophila</jats:italic> die when transferred at low density to a lipid‐free nutritionally complete medium. This death is prevented and they will start to proliferate if protein kinase C is activated and this activation is sustained. We propose that this takes place in two stages. Firstly, the phospholipase C pathway beginning with and specific for phosphatidylinositol leads to the formation of diacylglycerol and inositol <jats:italic>tris</jats:italic> ‐phosphate. Diacylglycerol activates protein kinase C, and inositol <jats:italic>tris</jats:italic> ‐phosphate via Ca<jats:sup>2+</jats:sup>phospholipase D (PLD). Secondly, the protein kinase C response can now be sustained by diacylglycerol produced by phospholipase D, using phosphatidylcholine and phosphatidylserine as substrates. Should this switching from PI‐specific phospholipase C (PLC) to phospholipase D fail, then the cell will die in the course of milliseconds during the minutes following inoculation.</jats:p>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10772772","pmcid":null,"openalex_id":"https://openalex.org/W2059819947","authors":[],"funders":[],"total_grants":0,"fwci":0.2638,"citation_percentile":0.63073852,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1006%2Fcbir.1999.0458","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1006/cbir.1999.0458","host_type":"publisher"},{"url":"https://doi.org/10.1006/cbir.1999.0458","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10772772","host_type":"repository"},{"url":"https://portal.findresearcher.sdu.dk/da/publications/d89ee640-6fce-11db-81a9-000ea68e967b","host_type":"repository"}],"fields_of_study":["Lipid metabolism and biosynthesis","Protist diversity and phylogeny","Cassava research and cyanide","Animals","Cell Death","Diglycerides","Models, Biological","Phosphatidylinositol 4,5-Diphosphate","Phosphatidylinositols","Phospholipase D","Signal Transduction","Temperature","Tetrahymena thermophila","Time Factors","Type C Phospholipases"],"mesh_terms":["Animals","Diglycerides","Models, Biological","Phosphatidylinositols","Type C Phospholipases","Phospholipase D","Temperature","Time Factors","Signal Transduction","Tetrahymena thermophila","Cell Death","Phosphatidylinositol 4,5-Diphosphate"],"keywords":["Diacylglycerol kinase","Phosphoinositide phospholipase C","Phospholipase C","Gq alpha subunit","Phospholipase","Protein kinase C","Phosphatidylinositol","Phospholipase D","Inositol","PLD2","Cell biology","Phosphatidylserine","Biochemistry","Kinase","Chemistry","Phosphatidylcholine","Biology","Signal transduction","Enzyme","Phospholipid","G protein","Receptor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T21:54:18.051541Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}