{"doi":"10.1006/bbrc.2002.6468","title":"A Discrete Domain of the Human TrkB Receptor Defines the Binding Sites for BDNF and NT-4","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2002,"id":622352,"datarank":0.5533319181170905,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"self_citation_contribution":0.5533319181170905,"citation_network_contribution":0.0,"self_endowment_contribution":0.5533319181170905,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1607959,"name":"Alan G.S Robertson","orcid":null,"position":1,"is_corresponding":false},{"id":1607960,"name":"Shelley J Allen","orcid":null,"position":2,"is_corresponding":false},{"id":1607961,"name":"Richard B Sessions","orcid":null,"position":3,"is_corresponding":false},{"id":1607962,"name":"Anthony R Clarke","orcid":null,"position":4,"is_corresponding":false},{"id":1607963,"name":"Grant G.F Mason","orcid":null,"position":5,"is_corresponding":false},{"id":1607964,"name":"Judy J Burston","orcid":null,"position":6,"is_corresponding":false},{"id":1607965,"name":"Sue J Tyler","orcid":null,"position":7,"is_corresponding":false},{"id":1607966,"name":"Gordon K Wilcock","orcid":null,"position":8,"is_corresponding":false},{"id":1607967,"name":"David Dawbarn","orcid":null,"position":9,"is_corresponding":false},{"id":1607958,"name":"Ruth L Naylor","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Discrete Domain of the Human TrkB Receptor Defines the Binding Sites for BDNF and NT-4","abstract":"TrkB is a member of the Trk family of tyrosine kinase receptors. In vivo, the extracellular region of TrkB is known to bind, with high affinity, the neurotrophin protein brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). We describe the expression and purification of the second Ig-like domain of human TrkB (TrkBIg(2)) and show, using surface plasmon resonance, that this domain is sufficient to bind BDNF and NT-4 with subnanomolar affinity. BDNF and NT-4 may have therapeutic implications for a variety of neurodegenerative diseases. The specificity of binding of the neurotrophins to their receptor TrkB is therefore of interest. We examine the specificity of TrkBIg(2) for all the neurotrophins, and use our molecular model of the BDNF-TrkBIg(2) complex to examine the residues involved in binding. It is hoped that the understanding of specific interactions will allow design of small molecule neurotrophin mimetics.","is_dataset_classified":null,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11855816","pmcid":null,"openalex_id":"https://openalex.org/W2007837302","authors":[],"funders":[],"total_grants":0,"fwci":0.5053,"citation_percentile":0.5355973,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2015,"count":4},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2023,"count":4},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X02964685?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X02964685?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.2002.6468","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11855816","host_type":"repository"},{"url":"https://research-information.bris.ac.uk/en/publications/d6e81485-2975-412f-94d8-41acc1f098e9","host_type":"repository"},{"url":"https://ora.ox.ac.uk/objects/uuid:8071e6e6-a4f4-46e9-bd63-59e6c6ec096b","host_type":"repository"},{"url":"https://hdl.handle.net/1983/d6e81485-2975-412f-94d8-41acc1f098e9","host_type":"repository"}],"fields_of_study":["Nerve injury and regeneration","Signaling Pathways in Disease","Virus-based gene therapy research","Amino Acid Sequence","Binding Sites","Brain-Derived Neurotrophic Factor","Circular Dichroism","Humans","Immunoglobulin Fragments","Kinetics","Models, Molecular","Molecular Sequence Data","Nerve Growth Factors","Protein Structure, Tertiary","Receptor, trkB","Sequence Homology, Amino Acid","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Surface Plasmon Resonance"],"mesh_terms":["Amino Acid Sequence","Binding Sites","Circular Dichroism","Humans","Immunoglobulin Fragments","Kinetics","Models, Molecular","Molecular Sequence Data","Nerve Growth Factors","Sequence Homology, Amino Acid","Protein Structure, Tertiary","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Brain-Derived Neurotrophic Factor","Surface Plasmon Resonance","Receptor, trkB"],"keywords":["Tropomyosin receptor kinase B","Neurotrophin","Trk receptor","Tropomyosin receptor kinase A","Brain-derived neurotrophic factor","Tropomyosin receptor kinase C","Neurotrophic factors","Low-affinity nerve growth factor receptor","Cell biology","Receptor","Biology","Neuroscience","Chemistry","Biochemistry","Growth factor","Platelet-derived growth factor receptor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T19:17:47.533348Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}