{"doi":"10.1006/bbrc.2001.5126","title":"Inhibition of Na,K-ATPase Activates PI3 Kinase and Inhibits Apoptosis in LLC-PK1 Cells","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2001,"id":654409,"datarank":0.6284482113039639,"base_score":4.189654742026425,"endowment":4.189654742026425,"self_citation_contribution":0.6284482113039639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6284482113039639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1707899,"name":"Guisen Jiang","orcid":null,"position":1,"is_corresponding":false},{"id":1707900,"name":"Aiping Zhao","orcid":null,"position":2,"is_corresponding":false},{"id":1707901,"name":"Tzvetanka Bondeva","orcid":null,"position":3,"is_corresponding":false},{"id":1707902,"name":"Przemyslaw Hirszel","orcid":null,"position":4,"is_corresponding":false},{"id":151850,"name":"Tamas Balla","orcid":null,"position":5,"is_corresponding":false},{"id":576834,"name":"Xiaoming Zhou","orcid":"0000-0003-4496-4445","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Inhibition of Na,K-ATPase Activates PI3 Kinase and Inhibits Apoptosis in LLC-PK1 Cells","abstract":"In the present study we used LLC-PK1 cells, a porcine renal proximal tubular cell line, to investigate whether PI3 kinase activation was involved in the anti-apoptotic effect of ouabain, a specific inhibitor of Na,K-ATPase. Apoptosis was induced by actinomycin D (Act D, 5 microM) and assessed by appearance of hypodiploid nuclei and DNA fragmentation. Ouabain attenuated Act D-induced apoptotic response in a dose-dependent manner. Incubation in a low K(+) medium (0.1 mM) which is another way to decrease Na,K-ATPase activity also had anti-apoptotic effect. Both ouabain and low K(+) medium increased the PI3 kinase activity in p85 immunoprecipitates. Ouabain, as well as incubation in the low K(+) medium, also increased the phosphorylation of Akt. Inhibition of PI3 kinase by either wortmannin or LY294002 reversed the cytoprotective effect of ouabain. These data together indicate that inhibition of Na,K-ATPase activates PI3 kinase in LLC-PK1 cells which could then exert the cytoprotective effect.","is_dataset_classified":null,"base_score":4.189654742026425,"endowment":4.189654742026425,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11437370","pmcid":null,"openalex_id":"https://openalex.org/W1993509170","authors":[],"funders":[],"total_grants":0,"fwci":2.0121,"citation_percentile":0.86138148,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":2},{"year":2023,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X01951265?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X01951265?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.2001.5126","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11437370","host_type":"repository"}],"fields_of_study":["Ion Transport and Channel Regulation","PI3K/AKT/mTOR signaling in cancer","Protein Kinase Regulation and GTPase Signaling","Animals","Apoptosis","Culture Media","Dactinomycin","Enzyme Activation","Enzyme Inhibitors","LLC-PK1 Cells","Ouabain","Phosphatidylinositol 3-Kinases","Phosphorylation","Protein Serine-Threonine Kinases","Proto-Oncogene Proteins","Proto-Oncogene Proteins c-akt","Sodium-Potassium-Exchanging ATPase","Swine"],"mesh_terms":["Sodium-Potassium-Exchanging ATPase","Animals","Culture Media","Dactinomycin","Enzyme Activation","Enzyme Inhibitors","Ouabain","Phosphorylation","Proto-Oncogene Proteins","Swine","Apoptosis","Protein Serine-Threonine Kinases","LLC-PK1 Cells","Phosphatidylinositol 3-Kinases","Proto-Oncogene Proteins c-akt"],"keywords":["Wortmannin","Ouabain","LY294002","Apoptosis","Kinase","Cell biology","Molecular biology","ATPase","Chemistry","DNA fragmentation","Phosphoinositide 3-kinase","Protein kinase B","Biology","Biochemistry","Enzyme","Programmed cell death","Sodium"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T06:28:54.573952Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}