{"doi":"10.1006/bbrc.2000.3673","title":"Reconstitution of Caspase-8 Sensitizes JB6 Cells to TRAIL","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":2000,"id":682240,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1782370,"name":"Chul-Woong Chung","orcid":null,"position":1,"is_corresponding":false},{"id":1558338,"name":"Ha-Na Woo","orcid":null,"position":2,"is_corresponding":false},{"id":1727040,"name":"Gil-Sun Hong","orcid":"0000-0002-0068-9413","position":3,"is_corresponding":false},{"id":1782371,"name":"Shinji Nagata","orcid":null,"position":4,"is_corresponding":false},{"id":1782372,"name":"Yong-Keun Jung","orcid":null,"position":5,"is_corresponding":false},{"id":1782368,"name":"In-Ki Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reconstitution of Caspase-8 Sensitizes JB6 Cells to TRAIL","abstract":"TRAIL induces apoptosis in various tumor cells. We report here that caspase-8 is required in TRAIL-induced cell death. Western blot analyses and enzyme assays showed that exposing Jurkat cells to TRAIL resulted in activation of caspases-8 followed by caspase-3 and -9. Acetyl-IETD-fluoromethylketone, a caspase-8 inhibitor, potently suppressed TRAIL-induced cell death compared to acetyl-DEVD-fluoromethylketone and acetyl-LEHD-fluoromethylketone, inhibitors of caspase-3 and caspase-9, respectively. JB6 cells, a caspase-8-deficient Jurkat variant, were completely resistant to TRAIL. However, reconstitution with a caspase-8, but not with caspase-2 or -3, sensitized JB6 cells to subsequent exposure to TRAIL. These results are indicative of the crucial function of caspase-8 in TRAIL-induced apoptosis in Jurkat cells.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11032723","pmcid":null,"openalex_id":"https://openalex.org/W2063791733","authors":[],"funders":[{"funder_name":"Ministry of Education","grant_id":"1998-D019-D00019","title":null},{"funder_name":"Korea Science and Engineering Foundation","grant_id":"","title":null}],"total_grants":2,"fwci":1.3442,"citation_percentile":0.80177595,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X00936738?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X00936738?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.2000.3673","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11032723","host_type":"repository"}],"fields_of_study":["Cell death mechanisms and regulation","Autophagy in Disease and Therapy","interferon and immune responses"],"mesh_terms":["Dose-Response Relationship, Drug","Enzyme Activation","Genes, Dominant","Humans","Membrane Glycoproteins","Mutation","Oligopeptides","Peptides","Plasmids","Recombinant Fusion Proteins","Time Factors","Tumor Cells, Cultured","Tumor Necrosis Factor-alpha","Blotting, Western","Cysteine Proteinase Inhibitors","Apoptosis","Jurkat Cells","Caspases","Apoptosis Regulatory Proteins","Caspase 3","Caspase 8","TNF-Related Apoptosis-Inducing Ligand","Caspase 9"],"keywords":["Jurkat cells","Caspase","Apoptosis","Western blot","Cell biology","Caspase 8","Programmed cell death","Caspase 3","Biology","Caspase 2","Molecular biology","Immunology","T cell","Biochemistry","Immune system"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T19:49:48.026374Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}