{"doi":"10.1006/bbrc.1999.1434","title":"Heparin Differentially Regulates the Interaction of Fibroblast Growth Factor-4 with FGF Receptors 1 and 2","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":1999,"id":590557,"datarank":1.6813096644219416,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":1.217653296418194,"self_endowment_contribution":0.4636563680037475,"citer_contribution":1.217653296418194,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":21,"citers_with_citation_signal":21,"citers_with_endowment":21,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1510979,"name":"Michal Safran","orcid":null,"position":1,"is_corresponding":false},{"id":1510980,"name":"Avner Yayon","orcid":null,"position":2,"is_corresponding":false},{"id":1510978,"name":"David Aviezer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Heparin Differentially Regulates the Interaction of Fibroblast Growth Factor-4 with FGF Receptors 1 and 2","abstract":"Fibroblast growth factor-4 (FGF4), like other FGFs, shares a high affinity for the anionic glycosaminoglycans heparin and heparan sulfate (HS), which in turn enhance FGF-receptor (FGFR) binding and activation. Here we demonstrate using a cell free system that, at low concentrations of heparin, FGF4 binds only to FGFR-2, while much higher heparin levels are required for binding to FGFR-1. Chemical crosslinking of radiolabeled FGF4 to the soluble FGF receptors confirms the preferential formation of FGF4-FGFR-2 complexes under restricted heparin availability, with maximal ligand-receptor interactions at almost 20-fold lower heparin concentrations then those required for the affinity labeling of FGFR-1. In accordance, HS-deficient cells expressing FGFR-2 proliferate in response to FGF4 at extremely low exogenous heparin concentrations, while FGFR-1 expressing cells are completely unresponsive under the same conditions. We suggest that FGFR-2 is the preferred receptor for FGF4 under restricted HS conditions and that the bioavailability of structurally distinct HS motifs may differentially control receptor specificity of FGF4 in vivo.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10512728","pmcid":null,"openalex_id":"https://openalex.org/W1981099772","authors":[],"funders":[],"total_grants":0,"fwci":0.3401,"citation_percentile":0.53383177,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2023,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X99914341?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X99914341?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.1999.1434","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10512728","host_type":"repository"}],"fields_of_study":["Fibroblast Growth Factor Research","Proteoglycans and glycosaminoglycans research","Advanced Fiber Laser Technologies"],"mesh_terms":["Affinity Labels","Cell Line","Cell-Free System","Chromatography, Affinity","Cross-Linking Reagents","Fibroblast Growth Factors","Heparin","Heparan Sulfate","Heparitin Sulfate","Kinetics","Ligands","Proto-Oncogene Proteins","Recombinant Proteins","Transfection","Receptors, Fibroblast Growth Factor","Receptor Protein-Tyrosine Kinases","Receptor, Fibroblast Growth Factor, Type 1","Receptor, Fibroblast Growth Factor, Type 2","Fibroblast Growth Factor 4"],"keywords":["Fibroblast growth factor receptor","Fibroblast growth factor","Receptor","Heparin","Chemistry","Fibroblast growth factor receptor 1","Biochemistry","Cell biology","Biology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-25T01:53:39.666537Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}