{"doi":"10.1006/bbrc.1994.1246","title":"Complete 5′ Noncoding Region Is Necessary for the Efficient Internal Initiation of Hepatitis C Virus RNA","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":1994,"id":588376,"datarank":5.682791714701994,"base_score":4.406719247264253,"endowment":4.406719247264253,"self_citation_contribution":0.6610078870896381,"citation_network_contribution":5.0217838276123565,"self_endowment_contribution":0.6610078870896381,"citer_contribution":5.0217838276123565,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":76,"citers_with_citation_signal":74,"citers_with_endowment":74,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1505240,"name":"K. Katayama","orcid":null,"position":1,"is_corresponding":false},{"id":1505241,"name":"C. Kurihara","orcid":null,"position":2,"is_corresponding":false},{"id":969590,"name":"N. Ishiyama","orcid":null,"position":3,"is_corresponding":false},{"id":1505242,"name":"F.B. Hoshino","orcid":null,"position":4,"is_corresponding":false},{"id":1458785,"name":"T. Ando","orcid":"0000-0003-4558-8904","position":5,"is_corresponding":false},{"id":1505245,"name":"A. Oya","orcid":null,"position":6,"is_corresponding":false},{"id":1505239,"name":"S. Fukushi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Complete 5′ Noncoding Region Is Necessary for the Efficient Internal Initiation of Hepatitis C Virus RNA","abstract":"The mechanism of translational initiation by the 5' noncoding region (5'NCR) of hepatitis C virus (HCV) genome was analyzed. Using an in vitro translation system with artificial RNA containing a modified 5' NCR of HCV under the various KCl conditions, nucleotides (nt.) 62 to 341 of the HCV 5'NCR were not functional as an internal ribosome entry site (IRES). However, the full-length 5'NCR (nt. 1 to 341) produced an efficient internal initiation. To identify the essential region of the HCV-IRES, various mutants were produced in which stem-loops, predicted by secondary structure analysis of the HCV 5'NCR, were deleted. These constructs were analyzed by in vitro translation. Comparison of translation efficiency among these mutants suggested that the alpha- or both alpha- and beta-branches of domain II are essential for efficient translation. Moreover, the formation of correct secondary structure of IRES seems to be stabilized by the presence of domain I in 5'NCR. Furthermore, the uncapped 5'NCR of HCV promotes translation more efficiently than capped truncated 5'NCR constructs. Our results strongly suggested that complete 5'NCR containing all stem-loop structures is necessary for initiation by HCV-IRES.","is_dataset_classified":null,"base_score":4.406719247264253,"endowment":4.406719247264253,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8135783","pmcid":null,"openalex_id":"https://openalex.org/W2051767229","authors":[],"funders":[],"total_grants":0,"fwci":3.6442,"citation_percentile":0.93386039,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X84712460?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X84712460?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.1994.1246","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8135783","host_type":"repository"}],"fields_of_study":["Hepatitis C virus research","Viral Infections and Immunology Research","RNA and protein synthesis mechanisms","Base Sequence","DNA Primers","Genome, Viral","Hepacivirus","Hepatitis C","Humans","Molecular Sequence Data","Nucleic Acid Conformation","Open Reading Frames","Peptide Chain Initiation, Translational","Polymerase Chain Reaction","Protein Biosynthesis","RNA Caps","RNA, Viral","Ribosomes"],"mesh_terms":["Base Sequence","Hepatitis C","Humans","Molecular Sequence Data","Nucleic Acid Conformation","Peptide Chain Initiation, Translational","Ribosomes","RNA Caps","RNA, Viral","Protein Biosynthesis","Polymerase Chain Reaction","Hepacivirus","Open Reading Frames","Genome, Viral","DNA Primers"],"keywords":["Internal ribosome entry site","Eukaryotic translation","Translation (biology)","RNA","Biology","Hepatitis C virus","Virology","Ribosome","Five prime untranslated region","Virus","Genetics","Chemistry","Computational biology","Cell biology","Messenger RNA","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-20T14:18:30.156870Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}