{"doi":"10.1006/bbrc.1993.2181","title":"The Presence of Ca2+-Independent Phospholipase-A1 Highly Specific for Phosphatidylinositol in Bovine Brain","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":1993,"id":653313,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1501200,"name":"T. Kobayashi","orcid":"0000-0003-3957-0839","position":1,"is_corresponding":false},{"id":1704613,"name":"M. Kishimoto","orcid":null,"position":2,"is_corresponding":false},{"id":1645968,"name":"T. Tsutsumi","orcid":null,"position":3,"is_corresponding":false},{"id":154581,"name":"S. Watanabe","orcid":"0009-0006-8308-3156","position":4,"is_corresponding":false},{"id":1704614,"name":"H. Okuyama","orcid":null,"position":5,"is_corresponding":false},{"id":205872,"name":"H. Ueda","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Presence of Ca2+-Independent Phospholipase-A1 Highly Specific for Phosphatidylinositol in Bovine Brain","abstract":"EDTA-insensitive phospholipase A activity hydrolyzing phosphatidylinositol was detected in a bovine brain soluble fraction. This phospholipase A was purified 25-fold by sequential chromatographies of DEAE-Toyopearl, Phenyl-Toyopearl, and Ultrahydrogel 1000. The partially purified EDTA-insensitive phospholipase A showed an apparent molecular mass of 230kDa on an Ultrahydrogel 1000 column in the presence of 0.05% Triton X-100 and a pH optimum at 7.0. The enzyme was highly specific for phosphatidylinositol; phosphatidylethanolamine and phosphatidylcholine were not hydrolyzed significantly. The enzyme activity was characterized as phospholipase A1, and Ca2+ and Mg2+ were not required for its activity. These results indicate the existence of Ca(2+)-independent, phosphatidylinositol-specific metabolism besides those catalyzed by Ca(2+)-dependent phospholipase A2 and Ca(2+)-dependent, phosphatidylinositol-specific phospholipase C.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8216258","pmcid":null,"openalex_id":"https://openalex.org/W2016066140","authors":[],"funders":[],"total_grants":0,"fwci":0.9456,"citation_percentile":0.72917787,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2022,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X83721819?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X83721819?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.1993.2181","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8216258","host_type":"repository"}],"fields_of_study":["Protein Kinase Regulation and GTPase Signaling","Cellular transport and secretion","Pancreatic function and diabetes","Animals","Brain","Calcium","Calcium Chloride","Cattle","Edetic Acid","Hydrolysis","Magnesium Chloride","Phosphatidylinositols","Phospholipases A","Phospholipases A1","Phospholipases A2","Substrate Specificity"],"mesh_terms":["Animals","Brain","Calcium","Calcium Chloride","Cattle","Edetic Acid","Hydrolysis","Phosphatidylinositols","Phospholipases A","Substrate Specificity","Magnesium Chloride","Phospholipases A1","Phospholipases A2"],"keywords":["Phosphatidylinositol","Phospholipase C","Phosphatidylethanolamine","Phosphatidylcholine","Phospholipase","Phospholipase D","Enzyme","Biochemistry","Chemistry","Hydrolysis","Chromatography","Phospholipid","Signal transduction","Membrane"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T20:46:24.021016Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}