{"doi":"10.1006/bbrc.1993.1242","title":"Protein Kinase C-α But Not Protein Kinase C-ϵ Is Differentially Down-Regulated by Bryostatin 1 and Tetradecanoyl Phorbol 13-Acetate in SH-SY5Y Human Neuroblastoma Cells","abstract":null,"journal":"Biochemical and Biophysical Research Communications","year":1993,"id":619968,"datarank":0.519860385419959,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.0,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1600213,"name":"M. Lintunen","orcid":null,"position":1,"is_corresponding":false},{"id":1600214,"name":"J. Heikkila","orcid":null,"position":2,"is_corresponding":false},{"id":1600212,"name":"A. Jalava","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Protein Kinase C-α But Not Protein Kinase C-ϵ Is Differentially Down-Regulated by Bryostatin 1 and Tetradecanoyl Phorbol 13-Acetate in SH-SY5Y Human Neuroblastoma Cells","abstract":"SH-SY5Y human neuroblastoma cells can be induced to differentiate by phorbol esters but not by bryostatins although both agents increase protein kinase C (PKC) activity in these cells to a similar extent. We examined whether this difference could be explained by differences in the responses of specific PKC isoenzymes. Both TPA and bryostatin 1 at 10 nM induced a rapid increase in membrane-associated PKC-alpha immunoreactivity which was sustained for 72 hours in TPA-treated cells, but was down-regulated within 24 hours in bryostatin-treated cells. TPA likewise induced a sustained phosphorylation of an 80 kDa PKC substrate whereas in bryostatin-treated cells the 80 kDa substrate was rapidly phosphorylated reaching a maximum at 6 hours followed by a decline to basal level within 48 hours. A higher concentration of TPA (300 nM), which results in a less differentiated phenotype, induced down-regulation of PKC-alpha within 24 hours. In contrast, both TPA and bryostatin 1 stimulated translocation and a partial down-regulation of PKC-epsilon with similar kinetics. These results suggest that the divergent actions of bryostatin 1 and TPA in SH-SY5Y cells are at least partially due to differential modulation of PKC-alpha but not PKC-epsilon by these two agents.","is_dataset_classified":null,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8461005","pmcid":null,"openalex_id":"https://openalex.org/W2092996522","authors":[],"funders":[],"total_grants":0,"fwci":3.1087,"citation_percentile":0.92234758,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2022,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0006291X83712428?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006291X83712428?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/bbrc.1993.1242","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8461005","host_type":"repository"}],"fields_of_study":["Neuroblastoma Research and Treatments","Protein Kinase Regulation and GTPase Signaling","Cardiac electrophysiology and arrhythmias","Biological Transport","Bryostatins","Cell Differentiation","Cytosol","Down-Regulation","Enzyme Activation","Humans","Isoenzymes","Lactones","Macrolides","Neuroblastoma","Phosphorylation","Protein Kinase C","Substrate Specificity","Tetradecanoylphorbol Acetate","Tumor Cells, Cultured"],"mesh_terms":["Biological Transport","Cell Differentiation","Cytosol","Enzyme Activation","Humans","Isoenzymes","Lactones","Neuroblastoma","Phosphorylation","Protein Kinase C","Substrate Specificity","Tetradecanoylphorbol Acetate","Tumor Cells, Cultured","Down-Regulation","Macrolides","Bryostatins"],"keywords":["Protein kinase C","Bryostatin 1","Phorbol","SH-SY5Y","Phosphorylation","Protein kinase A","Biology","Kinase","Molecular biology","In vitro","Neuroblastoma","Cell biology","Chemistry","Cell culture","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T09:25:49.877477Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}