{"doi":"10.1006/abbi.2002.2758","title":"Glucose Uptake and Lactate Production in Cells Exposed to CoCl2 and in Cells Overexpressing the Glut-1 Glucose Transporter","abstract":null,"journal":"Archives of Biochemistry and Biophysics","year":2002,"id":619320,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":597686,"name":"Faramarz Ismail-Beigi","orcid":null,"position":1,"is_corresponding":false},{"id":1598189,"name":"Daw-Yang Hwang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Glucose Uptake and Lactate Production in Cells Exposed to CoCl2 and in Cells Overexpressing the Glut-1 Glucose Transporter","abstract":"Glut-1-mediated glucose transport is augmented in response to a variety of conditions and stimuli. In this study we examined the metabolic fate of glucose in cells in which glucose transport is stimulated by exposure to CoCl(2), an agent that stimulates the expression of a set of hypoxia-responsive genes including several glycolytic enzymes and the Glut-1 glucose transporter. Similarly, we determined the metabolic fate of glucose in stably transfected cells overexpressing Glut-1. Exposure of Clone 9 liver cell line, 3T3-L1 fibroblasts, and C(2)C(12) myoblasts to CoCl(2) resulted in an increase glucose uptake and in the activity of glucose phosphorylation (\"hexokinase\") and lactate dehydrogenase. In cells treated with CoCl(2), the net increase in glucose taken up was accounted for by its near-complete conversion to lactate. Cells stably transfected to overexpress Glut-1 also exhibited enhanced net uptake of glucose with the near-complete conversion of the increased glucose taken up to lactate; however, the effect in these cells was observed in the absence of any change in the activity of two glycolytic enzymes examined. These findings suggest that in cells in which glucose transport is rate-limiting for glucose metabolism, enhancement of the glucose entry step per se results in a near-complete conversion of the extra glucose to lactate.","is_dataset_classified":null,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11888207","pmcid":null,"openalex_id":"https://openalex.org/W2032435579","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"R01-DK45945","title":null}],"total_grants":1,"fwci":0.2445,"citation_percentile":0.5604866,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S000398610292758X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S000398610292758X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/abbi.2002.2758","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11888207","host_type":"repository"}],"fields_of_study":["Cancer, Hypoxia, and Metabolism","Metabolism, Diabetes, and Cancer","Pancreatic function and diabetes"],"mesh_terms":["Animals","Cells, Cultured","Cobalt","Glucose","Monosaccharide Transport Proteins","3T3 Cells","Antimutagenic Agents","Lactic Acid","Glucose Transporter Type 1","Mice"],"keywords":["Glucose transporter","Hexokinase","Snf3","Glycolysis","Glucose uptake","Glucose Transporter Type 1","GLUT1","Carbohydrate metabolism","Transfection","Lactate dehydrogenase","Glucokinase","Metabolism","Cell culture","Biochemistry","Chemistry","Internal medicine","Enzyme","Biology","Endocrinology","Insulin","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T06:46:06.155094Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}