{"doi":"10.1002/yea.3537","title":"<i>CSU57</i> encodes a novel repressor of sorbose utilization in opportunistic human fungal pathogen <scp><i>Candida albicans</i></scp>","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:label/><jats:p>Human fungal pathogen <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans</jats:italic></jats:styled-content> cannot utilize L‐sorbose as a sole carbon source. However, chromosome 5 monosomic strains can grow on sorbose as repressors present on this chromosome get diminished allowing the expression of sorbose utilization gene (<jats:italic>SOU1</jats:italic>) located on chromosome 4. Functional identification of these repressors has been a difficult task as they are scattered on a large portion of the right arm of chromosome 5. Herein, we have applied the telomere‐mediated chromosomal truncation approach to identify a novel repressor for sorbose utilization in this pathogen. Multiple systematic chromosomal truncations were performed on the right arm of Chr5 in the background of <jats:italic>csu51</jats:italic>∆/<jats:italic>CSU51</jats:italic> to minimize the functional region to 6‐kb chromosomal stretch. Further, truncation that removes the part of <jats:italic>Orf19.3942</jats:italic> strongly suggested its role in sorbose utilization. However, compelling evidence comes from the observation that truncation at 1,044.288‐kb position of Chr5 in the strain <jats:italic>csu51</jats:italic>∆/<jats:italic>CSU51 orf19.3942</jats:italic>∆/<jats:italic>Orf.19.3942</jats:italic> produced Sou<jats:sup>+</jats:sup> phenotype; otherwise, the strain remains Sou<jats:sup>−</jats:sup>. This confirms beyond doubt the role of <jats:italic>Orf.19.3942</jats:italic> in the regulation of sorbose utilization and designated as <jats:italic>CSU57</jats:italic>. Comparison of <jats:italic>SOU1</jats:italic> gene expression of Sou<jats:sup>+</jats:sup> strains with wild type suggested its role at transcriptional level. Strain carrying double disruption of <jats:italic>CSU57</jats:italic> remains Sou<jats:sup>−</jats:sup>. Co‐overexpression of <jats:italic>SOU1</jats:italic> and <jats:italic>CSU57</jats:italic> together does not make the recipient strain Sou<jats:sup>−</jats:sup>; however, multiple tandem copies of <jats:italic>CSU57</jats:italic> produced diminished growth compared with control suggesting that it is a weak repressor. Taken together, we report that <jats:italic>CSU57</jats:italic> encodes a novel repressor of L‐sorbose utilization in this pathogen.</jats:p></jats:sec><jats:sec><jats:title>TAKE AWAY</jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>CSU57</jats:italic> encodes a repressor for L‐sorbose utilization in <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans</jats:italic></jats:styled-content>.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Csu57p acts in combination with Csu51p and other regulators.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Csu57p exerts its repressing effect at transcriptional level of <jats:italic>SOU1</jats:italic> gene.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Utilization of sorbose positively correlates to the expression of <jats:italic>SOU1</jats:italic> gene.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Multiple copies of <jats:italic>CSU57</jats:italic> can partially suppress Sou<jats:sup>+</jats:sup> phenotype.</jats:p></jats:list-item>\n</jats:list></jats:p></jats:sec>","journal":"Yeast","year":2021,"id":649577,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1693437,"name":"Dileep Pullepu","orcid":null,"position":1,"is_corresponding":false},{"id":1693438,"name":"Darshan Dhabalia","orcid":null,"position":2,"is_corresponding":false},{"id":1693439,"name":"Sagunthala Murugesan Udaya Prakash","orcid":null,"position":3,"is_corresponding":false},{"id":1693440,"name":"Mohammad Anaul Kabir","orcid":"0000-0003-0042-7795","position":4,"is_corresponding":false},{"id":1693436,"name":"Praveen Kumar Reddy","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<i>CSU57</i> encodes a novel repressor of sorbose utilization in opportunistic human fungal pathogen <scp><i>Candida albicans</i></scp>","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:label/><jats:p>Human fungal pathogen <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans</jats:italic></jats:styled-content> cannot utilize L‐sorbose as a sole carbon source. However, chromosome 5 monosomic strains can grow on sorbose as repressors present on this chromosome get diminished allowing the expression of sorbose utilization gene (<jats:italic>SOU1</jats:italic>) located on chromosome 4. Functional identification of these repressors has been a difficult task as they are scattered on a large portion of the right arm of chromosome 5. Herein, we have applied the telomere‐mediated chromosomal truncation approach to identify a novel repressor for sorbose utilization in this pathogen. Multiple systematic chromosomal truncations were performed on the right arm of Chr5 in the background of <jats:italic>csu51</jats:italic>∆/<jats:italic>CSU51</jats:italic> to minimize the functional region to 6‐kb chromosomal stretch. Further, truncation that removes the part of <jats:italic>Orf19.3942</jats:italic> strongly suggested its role in sorbose utilization. However, compelling evidence comes from the observation that truncation at 1,044.288‐kb position of Chr5 in the strain <jats:italic>csu51</jats:italic>∆/<jats:italic>CSU51 orf19.3942</jats:italic>∆/<jats:italic>Orf.19.3942</jats:italic> produced Sou<jats:sup>+</jats:sup> phenotype; otherwise, the strain remains Sou<jats:sup>−</jats:sup>. This confirms beyond doubt the role of <jats:italic>Orf.19.3942</jats:italic> in the regulation of sorbose utilization and designated as <jats:italic>CSU57</jats:italic>. Comparison of <jats:italic>SOU1</jats:italic> gene expression of Sou<jats:sup>+</jats:sup> strains with wild type suggested its role at transcriptional level. Strain carrying double disruption of <jats:italic>CSU57</jats:italic> remains Sou<jats:sup>−</jats:sup>. Co‐overexpression of <jats:italic>SOU1</jats:italic> and <jats:italic>CSU57</jats:italic> together does not make the recipient strain Sou<jats:sup>−</jats:sup>; however, multiple tandem copies of <jats:italic>CSU57</jats:italic> produced diminished growth compared with control suggesting that it is a weak repressor. Taken together, we report that <jats:italic>CSU57</jats:italic> encodes a novel repressor of L‐sorbose utilization in this pathogen.</jats:p></jats:sec><jats:sec><jats:title>TAKE AWAY</jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>CSU57</jats:italic> encodes a repressor for L‐sorbose utilization in <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans</jats:italic></jats:styled-content>.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Csu57p acts in combination with Csu51p and other regulators.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Csu57p exerts its repressing effect at transcriptional level of <jats:italic>SOU1</jats:italic> gene.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Utilization of sorbose positively correlates to the expression of <jats:italic>SOU1</jats:italic> gene.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Multiple copies of <jats:italic>CSU57</jats:italic> can partially suppress Sou<jats:sup>+</jats:sup> phenotype.</jats:p></jats:list-item>\n</jats:list></jats:p></jats:sec>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/yea.3537","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/yea.3537","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T03:57:14.098193Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}