{"doi":"10.1002/yea.1504","title":"Bimolecular fluorescence complementation analysis system for <i>in vivo</i> detection of protein–protein interaction in <i>Saccharomyces cerevisiae</i>","abstract":"<jats:title>Abstract</jats:title><jats:p>The bimolecular fluorescence complementation (BiFC) assay has been widely accepted for studying <jats:italic>in vivo</jats:italic> detection of protein–protein interactions in several organisms. To facilitate the application of the BiFC assay to yeast research, we have created a series of plasmids that allow single‐step, PCR‐based C‐ or N‐terminal tagging of yeast proteins with yellow fluorescent protein fragments for BiFC assay. By examination of several interacting proteins (Sis1–Sis1, Net1–Sir2, Cet1–Cet1 and Pho2–Pho4), we demonstrate that the BiFC assay can be used to reliably analyse the occurrence and subcellular localization of protein–protein interactions in living yeast cells. The sequences for the described plasmids were submitted to the GenBank under Accession Nos: EF210802, pFA6a‐VN‐His3MX6; EF210803, pFA6a‐VC‐His3MX6; EF210804, pFA6a‐VN‐TRP1; EF210807, pFA6a‐VC‐TRP1; EF210808, pFA6a‐VN‐kanMX6; EF210809, pFA6a‐VC‐kanMX6; EF210810, pFA6a‐His3MX6‐P<jats:sub>GAL1</jats:sub>‐VN; EF210805, pFA6a‐His3MX6‐P<jats:sub>GAL1</jats:sub>‐VC; EF210806, pFA6a‐TRP1‐P<jats:sub>GAL1</jats:sub>‐VN; EF210811, pFA6a‐TRP1‐P<jats:sub>GAL1</jats:sub>‐VC; EF210812, pFA6a‐kanMX6‐P<jats:sub>GAL1</jats:sub>‐VN; EF210813, pFA6a‐kanMX6‐P<jats:sub>GAL1</jats:sub>‐VC; EF521883, pFA6a‐His3MX6‐P<jats:sub>CET1</jats:sub>‐VN; EF521884, pFA6a‐His3MX6‐P<jats:sub>CET1</jats:sub>‐VC; EF521885, pFA6a‐TRP1‐P<jats:sub>CET1</jats:sub>‐VN; EF521886, pFA6a‐TRP1‐P<jats:sub>CET1</jats:sub>‐VC; EF521887, pFA6a‐kanMX6‐P<jats:sub>CET1</jats:sub>‐VN; EF521888, pFA6a‐kanMX6‐P<jats:sub>CET1</jats:sub>‐VC. Copyright © 2007 John Wiley &amp; Sons, Ltd.</jats:p>","journal":"Yeast","year":2007,"id":33689,"datarank":9.051421525097586,"base_score":5.262690188904886,"endowment":5.262690188904886,"self_citation_contribution":0.7894035283357329,"citation_network_contribution":8.262017996761854,"self_endowment_contribution":0.7894035283357329,"citer_contribution":8.262017996761854,"corpus_percentile":null,"corpus_rank":null,"citation_count":192,"citer_count":178,"citers_with_citation_signal":158,"citers_with_endowment":158,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":175776,"name":"Won‐Ki Huh","orcid":null,"position":1,"is_corresponding":false},{"id":175775,"name":"Min‐Kyung Sung","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.262690188904886,"endowment":5.262690188904886,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17534848","pmcid":null,"openalex_id":"https://openalex.org/W2160360032","authors":[],"funders":[],"total_grants":0,"fwci":3.498,"citation_percentile":0.93416317,"influential_citations":20,"citation_trend":[{"year":2012,"count":12},{"year":2013,"count":14},{"year":2014,"count":9},{"year":2015,"count":13},{"year":2016,"count":20},{"year":2017,"count":13},{"year":2018,"count":13},{"year":2019,"count":7},{"year":2020,"count":12},{"year":2021,"count":14},{"year":2022,"count":7},{"year":2023,"count":5},{"year":2024,"count":8},{"year":2025,"count":9},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fyea.1504","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/yea.1504","host_type":"publisher"},{"url":"https://doi.org/10.1002/yea.1504","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17534848","host_type":"repository"}],"fields_of_study":["Fungal and yeast genetics research","Endoplasmic Reticulum Stress and Disease","Biotin and Related Studies","Biology","Medicine","Bacterial Proteins","Base Sequence","DNA, Fungal","Genetic Vectors","Luminescent Proteins","Microscopy, Fluorescence","Molecular Sequence Data","Plasmids","Polymerase Chain Reaction","Protein Binding","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Sequence Analysis, DNA"],"mesh_terms":["Bacterial Proteins","Base Sequence","DNA, Fungal","Genetic Vectors","Luminescent Proteins","Microscopy, Fluorescence","Molecular Sequence Data","Plasmids","Protein Binding","Saccharomyces cerevisiae","Polymerase Chain Reaction","Sequence Analysis, DNA","Saccharomyces cerevisiae Proteins"],"keywords":["Bimolecular fluorescence complementation","Protein-fragment complementation assay","Biology","Saccharomyces cerevisiae","Yeast","Complementation","Plasmid","Molecular biology","Protein–protein interaction","Biochemistry","Gene","Phenotype"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T16:42:23.223732Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}