{"doi":"10.1002/stem.2220","title":"Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>MicroRNA (miRNAs) are short noncoding RNA molecules involved in many cellular processes and shown to play a key role in somatic cell induced reprogramming. We performed an array based screening to identify candidates that are differentially expressed between dermal skin fibroblasts (DFs) and induced pluripotent stem cells (iPSCs). We focused our investigations on miR-145 and showed that this candidate is highly expressed in DFs relative to iPSCs and significantly downregulated during reprogramming process. Inhibition of miR-145 in DFs led to the induction of “cellular plasticity” demonstrated by: (a) alteration of cell morphology associated with downregulation of mesenchymal and upregulation of epithelial markers; (b) upregulation of pluripotency-associated genes including SOX2, KLF4, C-MYC; (c) downregulation of miRNA let-7b known to inhibit reprogramming; and (iv) increased efficiency of reprogramming to iPSCs in the presence of reprogramming factors. Together, our results indicate a direct functional link between miR-145 and molecular pathways underlying reprogramming of somatic cells to iPSCs.</jats:p>","journal":"Stem Cells","year":2016,"id":21670,"datarank":1.2837171257071125,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.7420794388104788,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.7420794388104788,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":28,"citers_with_citation_signal":24,"citers_with_endowment":24,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":138711,"name":"Lucie Peskova","orcid":null,"position":1,"is_corresponding":false},{"id":138712,"name":"Joseph Collin","orcid":null,"position":2,"is_corresponding":false},{"id":138713,"name":"David Montaner","orcid":null,"position":3,"is_corresponding":false},{"id":138714,"name":"Irina Neganova","orcid":null,"position":4,"is_corresponding":false},{"id":138715,"name":"Lyle Armstrong","orcid":null,"position":5,"is_corresponding":false},{"id":138716,"name":"Majlinda Lako","orcid":null,"position":6,"is_corresponding":false},{"id":138710,"name":"Tomas Barta","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26418476","pmcid":"PMC4982107","openalex_id":"https://openalex.org/W2170207914","authors":[],"funders":[{"funder_name":"European Regional Development Fund - Project FNUSA-ICRC","grant_id":"CZ.1.05/1.1.00/02.0123","title":null},{"funder_name":"project ICRC-ERA-HumanBridge","grant_id":"316345","title":"Human Bridge for Strengthening Integration of ICRC into European Research Area"},{"funder_name":"7th Framework Programme of the European Union","grant_id":"MUNI/A/1558/2014","title":null},{"funder_name":"European STEMBANCC consortium and BBSRC UK","grant_id":"BB/I020209/1","title":"Mitochondrial function and transition from glycolysis to oxidative phosphorylation in differentiating induced pluripotent stem cells"},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/E012841/1","title":null},{"funder_name":"Medical Research Council","grant_id":"G0301182","title":null},{"funder_name":"European Commission","grant_id":"614620","title":"Exploiting the power of human induced pluripotent stem cells to generate synthetic retinae in vitro for cell based therapies, drug discovery and disease modelling"},{"funder_name":"Ministry of Education, Youth and Sports of the Czech Republic","grant_id":"","title":null}],"total_grants":8,"fwci":2.2586,"citation_percentile":0.88350171,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":5},{"year":2019,"count":4},{"year":2020,"count":10},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/stmcls/article-pdf/34/1/246/42023842/stmcls_34_1_246.pdf","host_type":"journal"},{"url":"https://academic.oup.com/stmcls/article-pdf/34/1/246/42023842/stmcls_34_1_246.pdf","host_type":"HYBRID"},{"url":"https://academic.oup.com/stmcls/article-pdf/34/1/246/42023842/stmcls_34_1_246.pdf","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fstem.2220","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/stem.2220","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/stem.2220","host_type":"publisher"},{"url":"https://doi.org/10.1002/stem.2220","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26418476","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4982107","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4982107","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4982107?pdf=render","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/pmc4982107?pdf=render","host_type":""},{"url":"http://dx.doi.org/10.1002/stem.2220","host_type":""},{"url":"https://fundanet.cipf.es/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2334","host_type":""},{"url":"https://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2334","host_type":""},{"url":"https://dx.doi.org/10.1002/stem.2220","host_type":""}],"fields_of_study":["Pluripotent Stem Cells Research","MicroRNA in disease regulation","RNA Interference and Gene Delivery","Biology","Medicine","0301 basic medicine","03 medical and health sciences","Base Sequence","Cellular Reprogramming","Dermis","Fibroblasts","Gene Expression Regulation","Humans","Induced Pluripotent Stem Cells","Kruppel-Like Factor 4","MicroRNAs","Molecular Sequence Data","Reproducibility of Results"],"mesh_terms":["Kruppel-Like Factor 4","Base Sequence","Fibroblasts","Gene Expression Regulation","Humans","Molecular Sequence Data","Reproducibility of Results","Dermis","MicroRNAs","Induced Pluripotent Stem Cells","Cellular Reprogramming"],"keywords":["Reprogramming","KLF4","Induced pluripotent stem cell","SOX2","Downregulation and upregulation","Biology","Cell biology","Somatic cell","Stem cell","microRNA","Cellular differentiation","Molecular biology","Cancer research","Cell","Embryonic stem cell","Genetics","Gene","c-myc","Induced Pluripotent Stem Cells","Oct4","Mesenchymal-to-epithelial Transition","Mir-145","Base Sequence","Molecular Sequence Data","Reproducibility of Results","Dermis","Fibroblasts","Cellular Reprogramming","Embryonic Stem Cells/Induced Pluripotent Stem Cells","Kruppel-Like Factor 4","MicroRNAs","Gene Expression Regulation","Humans"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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