{"doi":"10.1002/smll.202400485","title":"Dynamic Structures and Fast Transition Kinetics of Oxidized G‐Quadruplexes","abstract":"8-oxoguanines (8-oxoG) in cells form compromised G-quadruplexes (GQs), which may vary GQ mediated gene regulations. By mimicking molecularly crowded cellular environment using 40% DMSO or sucrose, here it is found that oxidized human telomeric GQs have stabilities close to the wild-type (WT) GQs. Surprisingly, while WT GQs show negative formation cooperativity between a Pt(II) binder and molecularly crowded environment, positive cooperativity is observed for oxidized GQ formation. Single-molecule mechanical unfolding reveals that 8-oxoG sequence formed more diverse and flexible structures with faster folding/unfolding transition kinetics, which facilitates the Pt(II) ligand to bind the best-fit structures with positive cooperativity. These findings offer new understanding on structures and properties of oxidized G-rich species in crowded environments. They also provide insights into the design of better ligands to target oxidized G-rich structures formed under oxidative cell stress.","journal":"Small","year":2024,"id":457544,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1283112,"name":"Arpit Sharma","orcid":"0000-0001-6912-8131","position":1,"is_corresponding":false},{"id":580565,"name":"Pravin Pokhrel","orcid":"0000-0003-4259-6087","position":2,"is_corresponding":false},{"id":420674,"name":"Deepak Karna","orcid":"0000-0003-3370-0997","position":3,"is_corresponding":false},{"id":773442,"name":"Shankar Pandey","orcid":"0000-0001-5576-6714","position":4,"is_corresponding":false},{"id":1283113,"name":"Yao‐Rong Zheng","orcid":"0000-0003-4605-1121","position":5,"is_corresponding":false},{"id":282461,"name":"Hanbin Mao","orcid":"0000-0002-6720-9429","position":6,"is_corresponding":false},{"id":1094157,"name":"Jiahao Ji","orcid":"0000-0002-6189-7750","position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-19T02:03:41.744499Z","pmid":"38678502","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}