{"doi":"10.1002/smll.202207805","title":"A Membrane‐Associated Light‐Harvesting Model is Enabled by Functionalized Assemblies of Gene‐Doubled TMV Proteins","abstract":"Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded within phospholipid membranes. Artificial light-harvesting models are valuable tools for understanding the structural features underpinning energy absorption and transfer within chromophore arrays. Here, a method for attaching a protein-based light-harvesting model to a planar, fluid supported lipid bilayer (SLB) is developed. The protein model consists of the tobacco mosaic viral capsid proteins that are gene-doubled to create a tandem dimer (dTMV). Assemblies of dTMV break the facial symmetry of the double disk to allow for differentiation between the disk faces. A single reactive lysine residue is incorporated into the dTMV assemblies for the site-selective attachment of chromophores for light absorption. On the opposing dTMV face, a cysteine residue is incorporated for the bioconjugation of a peptide containing a polyhistidine tag for association with SLBs. The dual-modified dTMV complexes show significant association with SLBs and exhibit mobility on the bilayer. The techniques used herein offer a new method for protein-surface attachment and provide a platform for evaluating excited state energy transfer events in a dynamic, fully synthetic artificial light-harvesting system.","journal":"Small","year":2023,"id":358232,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9479,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":510099,"name":"Kiera B. Wilhelm","orcid":"0000-0002-8781-7739","position":1,"is_corresponding":false},{"id":899676,"name":"Amanda J. Bischoff","orcid":"0000-0003-0802-275X","position":2,"is_corresponding":false},{"id":381723,"name":"J.H. Pereira","orcid":"0000-0002-8456-1774","position":3,"is_corresponding":false},{"id":1008105,"name":"Michel T. Dedeo","orcid":null,"position":4,"is_corresponding":false},{"id":829573,"name":"Derek García-Almedina","orcid":"0000-0002-0679-6930","position":5,"is_corresponding":false},{"id":39882,"name":"Paul D. Adams","orcid":"0000-0001-9333-8219","position":6,"is_corresponding":false},{"id":302378,"name":"Jay T. Groves","orcid":"0000-0002-3037-5220","position":7,"is_corresponding":false},{"id":284974,"name":"Matthew B. Francis","orcid":"0000-0003-2837-2538","position":8,"is_corresponding":false},{"id":422029,"name":"Jing Dai","orcid":"0000-0002-9819-0206","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T01:13:43.629667Z","pmid":"36811150","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}