{"doi":"10.1002/smll.202102567","title":"Label‐Free Identification of Single Mononucleotides by Nanoscale Electrophoresis","abstract":"Nanoscale electrophoresis allows for unique separations of single molecules, such as DNA/RNA nucleobases, and thus has the potential to be used as single molecular sensors for exonuclease sequencing. For this to be envisioned, label-free detection of the nucleotides to determine their electrophoretic mobility (i.e., time-of-flight, TOF) for highly accurate identification must be realized. Here, for the first time a novel nanosensor is shown that allows discriminating four 2-deoxyribonucleoside 5'-monophosphates, dNMPs, molecules in a label-free manner by nanoscale electrophoresis. This is made possible by positioning two sub-10 nm in-plane pores at both ends of a nanochannel column used for nanoscale electrophoresis and measuring the longitudinal transient current during translocation of the molecules. The dual nanopore TOF sensor with 0.5, 1, and 5 µm long nanochannel column lengths discriminates different dNMPs with a mean accuracy of 55, 66, and 94%, respectively. This nanosensor format can broadly be applicable to label-free detection and discrimination of other single molecules, vesicles, and particles by changing the dimensions of the nanochannel column and in-plane nanopores and integrating different pre- and postprocessing units to the nanosensor. This is simple to accomplish because the nanosensor is contained within a fluidic network made in plastic via replication.","journal":"Small","year":2021,"id":178771,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":724783,"name":"Zheng Jia","orcid":"0000-0003-2476-2969","position":1,"is_corresponding":false},{"id":724784,"name":"Ramin Riahipour","orcid":"0000-0002-3767-4266","position":2,"is_corresponding":false},{"id":598592,"name":"Collin McKinney","orcid":"0000-0002-7345-8325","position":3,"is_corresponding":false},{"id":724785,"name":"Charuni A. Amarasekara","orcid":"0000-0003-2566-8915","position":4,"is_corresponding":false},{"id":443733,"name":"Kumuditha M. Weerakoon‐Ratnayake","orcid":"0000-0002-6839-4515","position":5,"is_corresponding":false},{"id":275773,"name":"Steven A. Soper","orcid":"0000-0002-8292-7058","position":6,"is_corresponding":false},{"id":275772,"name":"Sunggook Park","orcid":"0000-0003-0424-6318","position":7,"is_corresponding":false},{"id":275771,"name":"Junseo Choi","orcid":"0000-0002-3461-3820","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-18T23:47:40.592580Z","pmid":"34558175","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}