{"doi":"10.1002/prot.26549","title":"Structure of a transmission blocking antibody in complex with Outer surface protein A from the Lyme disease spirochete, <i>Borreliella burgdorferi</i>","abstract":"319-44 is a human monoclonal antibody capable of passively protecting mice against tick-mediated infection with Borreliella burgdorferi, the bacterial genospecies responsible for Lyme disease in North America. In vitro, 319-44 has complement-dependent borreliacidal activity and spirochete agglutinating properties. Here, we report the 2.2 Å-resolution crystal structure of 319-44 Fab fragments in complex with Outer surface protein A (OspA), the ~30 kDa lipoprotein that was the basis of the first-generation Lyme disease vaccine approved in the United States. The 319-44 epitope is focused on OspA β-strands 19, 20, and 21, and the loops between β-strands 16-17, 18-19, and 20-21. Contact with loop 20-21 explains competition with LA-2, the murine monoclonal antibody used to estimate serum borreliacidal activities in the first-generation Lyme disease vaccine clinical trials. A high-resolution B-cell epitope map of OspA will accelerate structure-based design of second generation OspA-based vaccines.","journal":"Proteins Structure Function and Bioinformatics","year":2023,"id":348114,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9441,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":511324,"name":"Simon Davis","orcid":"0000-0001-7840-2411","position":1,"is_corresponding":false},{"id":1012255,"name":"H. M. Emranul Haque","orcid":null,"position":2,"is_corresponding":false},{"id":249880,"name":"Monir Ejemel","orcid":"0000-0001-9155-5914","position":3,"is_corresponding":false},{"id":249894,"name":"Lisa A. Cavacini","orcid":"0000-0003-1417-8339","position":4,"is_corresponding":false},{"id":430649,"name":"David J. Vance","orcid":"0000-0002-9913-7722","position":5,"is_corresponding":false},{"id":530241,"name":"Graham G. Willsey","orcid":"0000-0001-7595-5854","position":6,"is_corresponding":false},{"id":423478,"name":"Carol Lyn Piazza","orcid":null,"position":7,"is_corresponding":false},{"id":445965,"name":"David D. Weis","orcid":"0000-0003-3032-1211","position":8,"is_corresponding":false},{"id":249896,"name":"Yang Wang","orcid":"0000-0002-5554-8247","position":9,"is_corresponding":false},{"id":45735,"name":"Nicholas J. Mantis","orcid":"0000-0002-5083-8640","position":10,"is_corresponding":false},{"id":511323,"name":"M. Rudolph","orcid":"0000-0002-5893-9817","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-19T01:12:06.040073Z","pmid":"37455569","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}