{"doi":"10.1002/prot.24804","title":"Structural analysis of the polo‐box domain of human Polo‐like kinase 2","abstract":"<jats:title>ABSTRACT</jats:title><jats:p>Polo‐like kinases (Plks) are the key regulators of cell cycle progression, the members of which share a kinase domain and a polo‐box domain (PBD) that serves as a protein‐binding module. While Plk1 is a promising target for antitumor therapy, Plk2 is regarded as a tumor suppressor even though the two Plks commonly recognize the S‐pS/T‐P motif through their PBD. Herein, we report the crystal structure of the PBD of Plk2 at 2.7 Å. Despite the overall structural similarity with that of Plk1 reflecting their high sequence homology, the crystal structure also contains its own features including the highly ordered loop connecting two subdomains and the absence of 3<jats:sub>10</jats:sub>‐helices in the N‐terminal region unlike the PBD of Plk1. Based on the three‐dimensional structure, we furthermore could model its interaction with two types of phosphopeptides, one of which was previously screened as the optimal peptide for the PBD of Plk2. Proteins 2015; 83:1201–1208. © 2015 Wiley Periodicals, Inc.</jats:p>","journal":"Proteins: Structure, Function, and Bioinformatics","year":2015,"id":44163,"datarank":0.7804984386559053,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.3957560350366748,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.3957560350366748,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":10,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":208402,"name":"Bonsu Ku","orcid":null,"position":1,"is_corresponding":false},{"id":205179,"name":"Kyung S. Lee","orcid":null,"position":2,"is_corresponding":false},{"id":69255,"name":"Seung Jun Kim","orcid":null,"position":3,"is_corresponding":false},{"id":208401,"name":"Ju Hee Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25846005","pmcid":"PMC7720676","openalex_id":"https://openalex.org/W1950033056","authors":[],"funders":[{"funder_name":"National Research Foundation of Korea","grant_id":"2011-0030027","title":null},{"funder_name":"Intramural NIH HHS","grant_id":"ZIA BC011324","title":null},{"funder_name":"Korea Research Institute of Bioscience and Biotechnology Research Initiative Program","grant_id":"","title":null}],"total_grants":3,"fwci":0.7849,"citation_percentile":0.69877033,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2017,"count":1},{"year":2018,"count":3},{"year":2019,"count":1},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"green","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7720676","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720676","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7720676","host_type":"repository"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fprot.24804","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/prot.24804","host_type":"publisher"},{"url":"https://doi.org/10.1002/prot.24804","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25846005","host_type":"repository"},{"url":"http://doi.org/10.1002/prot.24804","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Cancer-related Molecular Pathways","DNA Repair Mechanisms","Medicine","Biology","Chemistry","Amino Acid Motifs","Binding Sites","Cell Cycle Proteins","Cloning, Molecular","Crystallography, X-Ray","Escherichia coli","Gene Expression","Humans","Hydrogen Bonding","Hydrophobic and Hydrophilic Interactions","Models, Molecular","Molecular Sequence Data","Phosphopeptides","Protein Binding","Protein Serine-Threonine Kinases","Protein Structure, Secondary","Protein Structure, Tertiary","Proto-Oncogene Proteins","Recombinant Proteins","Structural Homology, Protein","Polo-Like Kinase 1"],"mesh_terms":["Polo-Like Kinase 1","Binding Sites","Cloning, Molecular","Escherichia coli","Humans","Hydrogen Bonding","Models, Molecular","Molecular Sequence Data","Phosphopeptides","Protein Binding","Proto-Oncogene Proteins","Recombinant Proteins","Gene Expression","Protein Serine-Threonine Kinases","Protein Structure, Secondary","Protein Structure, Tertiary","Crystallography, X-Ray","Cell Cycle Proteins","Amino Acid Motifs","Structural Homology, Protein","Hydrophobic and Hydrophilic Interactions"],"keywords":["Polo-like kinase","PLK1","Kinase","Protein kinase domain","Structural similarity","Chemistry","Cell biology","Computational biology","Biology","Biochemistry","Cell cycle","Cell","Structure","Tumor suppressor","Polo-box Domain","Polo-like Kinase 2"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-16T17:24:34.261143Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}