{"doi":"10.1002/pro.4919","title":"Cell surface β‐lactamase recruitment: A facile selection to identify protein–protein interactions","abstract":"Protein-protein interactions (PPIs) are central to many cellular processes, and the identification of novel PPIs is a critical step in the discovery of protein therapeutics. Simple methods to identify naturally existing or laboratory evolved PPIs are therefore valuable research tools. We have developed a facile selection that links PPI-dependent β-lactamase recruitment on the surface of Escherichia coli with resistance to ampicillin. Bacteria displaying a protein that forms a complex with a specific protein-β-lactamase fusion are protected from ampicillin-dependent cell death. In contrast, bacteria that do not recruit β-lactamase to the cell surface are killed by ampicillin. Given its simplicity and tunability, we anticipate this selection will be a valuable addition to the palette of methods for illuminating and interrogating PPIs.","journal":"Protein Science","year":2024,"id":483177,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9563,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1190056,"name":"Jade Mia King","orcid":null,"position":1,"is_corresponding":false},{"id":1190057,"name":"Latrina J. Mayo","orcid":null,"position":2,"is_corresponding":false},{"id":1190058,"name":"Cierra R. Faries","orcid":null,"position":3,"is_corresponding":false},{"id":1324411,"name":"Ya'hnis T. Lockett","orcid":null,"position":4,"is_corresponding":false},{"id":710399,"name":"David W. Crawford","orcid":"0000-0003-2948-1007","position":5,"is_corresponding":false},{"id":1076735,"name":"Patrick C. Beardslee","orcid":"0000-0002-8847-3333","position":6,"is_corresponding":false},{"id":430614,"name":"Alexander Hendricks","orcid":"0009-0000-1309-0453","position":7,"is_corresponding":false},{"id":575663,"name":"Brian R. McNaughton","orcid":"0000-0002-9931-0361","position":8,"is_corresponding":false},{"id":1190055,"name":"Jordan A. Hinmon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":null,"created_at":"2026-07-19T02:07:22.248464Z","pmid":"38501433","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}