{"doi":"10.1002/prca.202300128","title":"A novel micropeptide, <i>Slitharin</i>, exerts cardioprotective effects in myocardial infarction","abstract":"PURPOSE: Micropeptides are an emerging class of proteins that play critical roles in cell signaling. Here, we describe the discovery of a novel micropeptide, dubbed slitharin (Slt), in conditioned media from Cardiosphere-derived cells (CDCs), a therapeutic cardiac stromal cell type. EXPERIMENTAL DESIGN: We performed mass spectrometry of peptide-enriched fractions from the conditioned media of CDCs and a therapeutically inert cell type (human dermal fibrobasts). We then evaluated the therapeutic capacity of the candidate peptide using an in vitro model of cardiomyocyte injury and a rat model of myocardial infarction. RESULTS: We identified a novel 24-amino acid micropeptide (dubbed Slitharin [Slt]) with a non-canonical leucine start codon, arising from long intergenic non-coding (LINC) RNA 2099. Neonatal rat ventricular myocytes (NRVMs) exposed to Slt were protected from hypoxic injury in vitro compared to a vehicle or scrambled control. Transcriptomic analysis of cardiomyocytes exposed to Slt reveals cytoprotective capacity, putatively through regulation of stress-induced MAPK-ERK. Slt also exerted cardioprotective effects in rats with myocardial infarction as shown by reduced infarct size 48 h post-injury. Conclusions and clinical relavance: Thus, Slt is a non-coding RNA-derived micropeptide, identified in the extracellular space, with a potential cardioprotective function.","journal":"PROTEOMICS - CLINICAL APPLICATIONS","year":2024,"id":464983,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9654,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":465877,"name":"Alessandra Ciullo","orcid":"0000-0002-8681-3883","position":1,"is_corresponding":false},{"id":1296146,"name":"Shukuro Yamaguchi","orcid":"0000-0002-5320-6366","position":2,"is_corresponding":false},{"id":1296147,"name":"Chang Li","orcid":"0000-0003-3252-7360","position":3,"is_corresponding":false},{"id":715899,"name":"Travis J. Antes","orcid":"0000-0002-2294-8797","position":4,"is_corresponding":false},{"id":953964,"name":"Xaviar M Jones","orcid":"0000-0003-1815-4166","position":5,"is_corresponding":false},{"id":1022244,"name":"Liang Li","orcid":"0000-0003-0745-2571","position":6,"is_corresponding":false},{"id":663823,"name":"Ramachandran Murali","orcid":"0000-0002-8384-2793","position":7,"is_corresponding":false},{"id":878455,"name":"Innokentiy Maslennikov","orcid":"0000-0003-4292-5821","position":8,"is_corresponding":false},{"id":471722,"name":"Niveda Sundararaman","orcid":"0000-0001-7708-3754","position":9,"is_corresponding":false},{"id":582572,"name":"Daniel Soetkamp","orcid":"0000-0001-8276-4175","position":10,"is_corresponding":false},{"id":317255,"name":"Eugenio Cingolani","orcid":"0000-0001-5939-8111","position":11,"is_corresponding":false},{"id":112291,"name":"Jennifer E. Van Eyk","orcid":"0000-0001-9050-148X","position":12,"is_corresponding":false},{"id":108831,"name":"Eduardo Marbán","orcid":"0000-0002-3863-280X","position":13,"is_corresponding":false},{"id":644329,"name":"Ahmed Ibrahim","orcid":"0000-0002-1657-7432","position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":null,"created_at":"2026-07-19T02:04:46.201722Z","pmid":"38444254","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}