{"doi":"10.1002/pmic.202100231","title":"Quantitative proteomics revealed new functions of ALKBH4","abstract":"Abstract ALKBH4 is a versatile demethylase capable of catalyzing the demethylation of monomethylated lysine‐84 on actin and N 6 ‐methyladenine in DNA. In this study, we conducted a quantitative proteomic experiment to reveal the altered expression of proteins in HEK293T cells upon genetic ablation of ALKBH4 . Our results showed markedly diminished levels of GSTP1 and HSPB1 proteins in ALKBH4‐depleted cells, which emanate from an augmented expression level of DNA (cytosine‐5)‐methyltransferase 1 (DNMT1) and the ensuing elevated cytosine methylation in the promoter regions of GSTP1 and HSPB1 genes. Together, our results revealed a role of ALKBH4 in modulating DNA cytosine methylation through regulating the expression level of DNMT1 protein.","journal":"PROTEOMICS","year":2021,"id":196711,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":446227,"name":"Tianyu F. Qi","orcid":null,"position":1,"is_corresponding":false},{"id":17757,"name":"Weili Miao","orcid":"0000-0002-7487-8962","position":2,"is_corresponding":false},{"id":269776,"name":"Xiaochuan Liu","orcid":"0000-0003-0444-420X","position":3,"is_corresponding":false},{"id":110450,"name":"Yinsheng Wang","orcid":"0000-0001-5565-283X","position":4,"is_corresponding":false},{"id":769936,"name":"Kailin Yu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":null,"created_at":"2026-07-18T23:50:15.704473Z","pmid":"34951099","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}