{"doi":"10.1002/pmic.202000279","title":"Mass spectrometry‐based proteomic platforms for better understanding of SARS‐CoV‐2 induced pathogenesis and potential diagnostic approaches","abstract":"While protein-protein interaction is the first step of the SARS-CoV-2 infection, recent comparative proteomic profiling enabled the identification of over 11,000 protein dynamics, thus providing a comprehensive reflection of the molecular mechanisms underlying the cellular system in response to viral infection. Here we summarize and rationalize the results obtained by various mass spectrometry (MS)-based proteomic approaches applied to the functional characterization of proteins and pathways associated with SARS-CoV-2-mediated infections in humans. Comparative analysis of cell-lines versus tissue samples indicates that our knowledge in proteome profile alternation in response to SARS-CoV-2 infection is still incomplete and the tissue-specific response to SARS-CoV-2 infection can probably not be recapitulated efficiently by in vitro experiments. However, regardless of the viral infection period, sample types, and experimental strategies, a thorough cross-comparison of the recently published proteome, phosphoproteome, and interactome datasets led to the identification of a common set of proteins and kinases associated with PI3K-Akt, EGFR, MAPK, Rap1, and AMPK signaling pathways. Ephrin receptor A2 (EPHA2) was identified by 11 studies including all proteomic platforms, suggesting it as a potential future target for SARS-CoV-2 infection mechanisms and the development of new therapeutic strategies. We further discuss the potentials of future proteomics strategies for identifying prognostic SARS-CoV-2 responsive age-, gender-dependent, tissue-specific protein targets.","journal":"PROTEOMICS","year":2021,"id":160297,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9404,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":443272,"name":"R. Shyama Prasad Rao","orcid":"0000-0002-2285-6788","position":1,"is_corresponding":false},{"id":377917,"name":"Rashaun S. Wilson","orcid":null,"position":2,"is_corresponding":false},{"id":673348,"name":"Ujwal Punyamurtula","orcid":null,"position":3,"is_corresponding":false},{"id":637784,"name":"Fernanda Salvato","orcid":"0000-0003-1650-5399","position":4,"is_corresponding":false},{"id":672780,"name":"Max Petersen","orcid":"0000-0003-3179-4619","position":5,"is_corresponding":false},{"id":673349,"name":"Mohammad Kabir Ahmed","orcid":null,"position":6,"is_corresponding":false},{"id":373709,"name":"M. Ruhul Abid","orcid":"0000-0003-2981-2638","position":7,"is_corresponding":false},{"id":631830,"name":"Jacob Verburgt","orcid":"0000-0002-8524-1342","position":8,"is_corresponding":false},{"id":316939,"name":"Daisuke Kihara","orcid":"0000-0003-4091-6614","position":9,"is_corresponding":false},{"id":311990,"name":"Zhibo Yang","orcid":"0000-0003-0370-7450","position":10,"is_corresponding":false},{"id":267526,"name":"Luca Fornelli","orcid":"0000-0001-6334-1046","position":11,"is_corresponding":false},{"id":673350,"name":"Steven B. Foster","orcid":null,"position":12,"is_corresponding":false},{"id":405566,"name":"Bharat Ramratnam","orcid":null,"position":13,"is_corresponding":false},{"id":568422,"name":"Nagib Ahsan","orcid":"0000-0002-6813-7527","position":0,"is_corresponding":true}],"reference_count":102,"raw_metadata":null,"created_at":"2026-07-18T23:44:43.908634Z","pmid":"33860983","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}