{"doi":"10.1002/pld3.530","title":"Simultaneous CAS9 editing of cp<i>SRP43</i>, <i>LHCA6</i>, and <i>LHCA7</i> in <i>Picochlorum celeri</i> lowers chlorophyll levels and improves biomass productivity","abstract":"Abstract High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri , CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (&gt;50% lower chlorophyll) were generated, with proteomics showing attenuated levels of most light harvesting complex (LHC) proteins. Gene editing generated two types of cpSRP43 transformants with distinct lower pigment phenotypes: (i) a transformant (Δ srp43 ) with both cp SRP43 diploid alleles modified to encode non‐functional polypeptides and (ii) a transformant (STR30309) with a 3 nt in‐frame insertion in one allele at the CAS9 cut site (non‐functional second allele), leading to expression of a modified cpSRP43. STR30309 has more chlorophyll than Δ srp43 but substantially less than wild type. To further decrease light absorption by photosystem I in STR30309, CAS9 editing was used to stack in disruptions of both LHCA6 and LHCA7 to generate STR30843, which has higher (5–24%) productivities relative to wild type in solar‐simulating bioreactors. Maximal productivities required frequent partial harvests throughout the day. For STR30843, exemplary diel bioreactor yields of ~50 g m −2 day −1 were attained. Our results demonstrate diel productivity gains in P. celeri by lowering pigment levels.","journal":"Plant Direct","year":2023,"id":341628,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":656824,"name":"Mélissa Cano","orcid":"0000-0002-5428-9642","position":1,"is_corresponding":false},{"id":1077576,"name":"Devin A.J. Karns","orcid":"0000-0002-9360-5329","position":2,"is_corresponding":false},{"id":1077577,"name":"Tyson A. Burch","orcid":"0000-0001-8999-4950","position":3,"is_corresponding":false},{"id":657705,"name":"Maria Likhogrud","orcid":null,"position":4,"is_corresponding":false},{"id":1078169,"name":"Moena Aqui","orcid":null,"position":5,"is_corresponding":false},{"id":1078170,"name":"Shaun Bailey","orcid":null,"position":6,"is_corresponding":false},{"id":1078171,"name":"John Verruto","orcid":null,"position":7,"is_corresponding":false},{"id":661983,"name":"W. Marcus Lambert","orcid":"0000-0002-4592-5176","position":8,"is_corresponding":false},{"id":1078172,"name":"Fedor I. Kuzminov","orcid":null,"position":9,"is_corresponding":false},{"id":1078173,"name":"Mahva Naghipor","orcid":null,"position":10,"is_corresponding":false},{"id":699509,"name":"Yingjun Wang","orcid":"0000-0002-6332-9706","position":11,"is_corresponding":false},{"id":550588,"name":"Christopher C. Ebmeier","orcid":"0000-0001-7940-6190","position":12,"is_corresponding":false},{"id":656828,"name":"Joseph C. Weissman","orcid":"0000-0002-6278-4280","position":13,"is_corresponding":false},{"id":656829,"name":"Matthew C. Posewitz","orcid":"0000-0002-1163-8611","position":14,"is_corresponding":false},{"id":656823,"name":"Anagha Krishnan","orcid":"0000-0002-0044-271X","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T01:11:08.077724Z","pmid":"37711644","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}