{"doi":"10.1002/oby.22722","title":"MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome","abstract":"OBJECTIVE: Adipose tissue plays a key role in obesity-related metabolic dysfunction. MicroRNA (miRNA) are gene regulatory molecules involved in intercellular and inter-organ communication. It was hypothesized that miRNA levels in adipose tissue would change after gastric bypass surgery and that this would provide insights into their role in obesity-induced metabolic dysregulation. METHODS: miRNA profiling (Affymetrix GeneChip miRNA 2.0 Array) of omental and subcutaneous adipose (n = 15 females) before and after gastric bypass surgery was performed. RESULTS: One omental and thirteen subcutaneous adipose miRNAs were significantly differentially expressed after gastric bypass, including downregulation of miR-223-3p and its antisense relative miR-223-5p in both adipose tissues. mRNA levels of miR-223-3p targets NLRP3 and GLUT4 were decreased and increased, respectively, following gastric bypass in both adipose tissues. Significantly more NLRP3 protein was observed in omental adipose after gastric bypass (P = 0.02). Significant hypomethlyation of NLRP3 and hypermethylation of miR-223 were observed in both adipose tissues after gastric bypass. In subcutaneous adipose, significant correlations were observed between both miR-223-3p and miR-223-5p and glucose and between NLRP3 mRNA and protein levels and blood lipids. CONCLUSIONS: This is the first report detailing genome-wide miRNA profiling of omental adipose before and after gastric bypass, and it further highlights the association of miR-223-3p and the NLRP3 inflammasome with obesity.","journal":"Obesity","year":2020,"id":75647,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8944,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395060,"name":"Kirsty Danielson","orcid":"0000-0002-1667-2528","position":1,"is_corresponding":false},{"id":396307,"name":"Jane Clapham","orcid":null,"position":2,"is_corresponding":false},{"id":395061,"name":"Miles C. Benton","orcid":"0000-0003-3442-965X","position":3,"is_corresponding":false},{"id":395062,"name":"A.C. Johnstone","orcid":"0000-0003-0736-9751","position":4,"is_corresponding":false},{"id":86840,"name":"Angela L. Jones","orcid":"0000-0002-1565-4360","position":5,"is_corresponding":false},{"id":395063,"name":"Odette M. Shaw","orcid":"0000-0001-7158-1074","position":6,"is_corresponding":false},{"id":396308,"name":"Ronald Hagan","orcid":null,"position":7,"is_corresponding":false},{"id":88823,"name":"Eric P. Hoffman","orcid":"0000-0001-6470-5139","position":8,"is_corresponding":false},{"id":395064,"name":"Mark Hayes","orcid":"0000-0002-5665-2863","position":9,"is_corresponding":false},{"id":396309,"name":"Jacquie L. Harper","orcid":null,"position":10,"is_corresponding":false},{"id":395065,"name":"Michael A. Langston","orcid":"0000-0001-5945-5796","position":11,"is_corresponding":false},{"id":396310,"name":"Richard S. Stubbs","orcid":null,"position":12,"is_corresponding":false},{"id":395059,"name":"Donia Macartney‐Coxson","orcid":"0000-0002-1051-537X","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-18T21:46:55.856612Z","pmid":"32090515","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}