{"doi":"10.1002/nbm.70014","title":"Deuterium MRS for In Vivo Measurement of Lipogenesis in the Liver","abstract":"ABSTRACT Hepatic de novo lipogenesis (DNL) plays a key role in the pathogenesis of several metabolic diseases that affect the liver. In humans, the detection of deuterium ( 2 H) in triglycerides from very low density lipoprotein collected from blood after administration of deuterated water (D 2 O) is commonly used as an indirect estimate of hepatic DNL. Here, we tested in rats (1) the feasibility to detect 2 H‐labeling directly in liver lipids in vivo by using noninvasive 2 H MRS and (2) to what extent these results correlated with the gold standard measurement of DNL in excised liver tissue. To increase hepatic DNL, half of the animals ( n = 4) underwent a 7‐week dietary intervention in which fructose was provided in drinking water. Deuterium MRS data were acquired from a single voxel placed in the liver. In vivo 2 H MRS data showed 2 H‐labeling in the combined peak of methyl and methylene resonances after 1 week of administrati NBM_70014 on of 5% D 2 O as drinking water. DNL was calculated using 1 H and 2 H NMR data acquired from extracted lipids of excised liver tissue. The 2 H lipid level measured in vivo correlated with the ex vivo estimates of hepatic DNL ( r = 0.81, p = 0.016). These results demonstrate the feasibility of direct detection of deuterium labeling in liver lipids using localized 2 H MRS in vivo and indicate the potential of this approach to measure hepatic DNL. These initial observations provide a basis for the method to be translated and to develop noninvasive, quantitative measurements of hepatic DNL in humans.","journal":"NMR in Biomedicine","year":2025,"id":540467,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9575,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":262947,"name":"Robin A. de Graaf","orcid":"0000-0002-6570-7010","position":1,"is_corresponding":false},{"id":994135,"name":"Monique Thomas","orcid":null,"position":2,"is_corresponding":false},{"id":1428213,"name":"Jeanine J. Prompers","orcid":"0000-0002-4756-4474","position":3,"is_corresponding":false},{"id":535240,"name":"Henk M. De Feyter","orcid":"0000-0001-6111-0316","position":4,"is_corresponding":false},{"id":1428212,"name":"Ayhan Gürsan","orcid":"0000-0002-8862-3012","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:52:38.861025Z","pmid":"39994887","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}