{"doi":"10.1002/nau.24896","title":"The preventive effects of colony‐stimulating factor 1 receptor (CSF‐1R) inhibition on bladder outlet obstruction induced remodeling","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Introduction</jats:title><jats:p>Bladder outlet obstruction (BOO) is a common problem that can affect bladder structure and function. Currently, there is no effective drugs available to prevent BOO‐induced remodeling. Previous reports have demonstrated that the pathogenesis of BOO is associated with macrophage infiltration and polarization, which is physiologically dependent on colony‐stimulating factor 1 receptor (CSF‐1R) activation. Here we utilized a highly selective CSF‐1R inhibitor, GW2580, to determine its preventive effects on BOO‐induced remodeling.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A total of 24 Sprague–Dawley rats were randomly divided into sham, BOO + vehicle, and BOO + GW2580 group. GW2580 or vehicle control was administrated by oral gavage at daily doses of 40 mg/kg for 6 weeks. Bladder samples were collected for histopathology, immunohistochemistry, immunofluorescence, western blotting, and flow cytometry analysis.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Our results demonstrated that bladder fibrosis was ameliorated by GW2580 compared with the vehicle group (22.01% ± 5.13% vs. 32.15% ± 7.24%, <jats:italic>p</jats:italic> &lt; 0.01). Furthermore, treatment with GW2580 induced an inhibition of macrophage infiltration (4.41% ± 1.28% vs. 13.57% ± 3.42%, <jats:italic>p</jats:italic> &lt; 0.001) and M2 macrophage polarization (10.67% ± 4.15% vs. 28.59% ± 6.38%, <jats:italic>p</jats:italic> &lt; 0.001). There was also a decrease of profibrotic F4/80<jats:sup>+</jats:sup> α‐smooth muscle actin<jats:sup>+</jats:sup> (α‐SMA<jats:sup>+</jats:sup>) macrophage to myofibroblast transition (9.11% ± 2.58% vs. 17.33% ± 4.01%, <jats:italic>p</jats:italic> &lt; 0.001) and CD163<jats:sup>+</jats:sup>TGF‐β1<jats:sup>+</jats:sup> cells (7.68% ± 2.10% vs. 14.17% ± 4.09%, <jats:italic>p</jats:italic> &lt; 0.01) in the GW2580 group when compared with the vehicle group.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In summary, our findings showed that GW2580 is a worthwhile candidate for a follow‐up study to test in the treatment of BOO‐induced remodeling.</jats:p></jats:sec>","journal":"Neurourology and Urodynamics","year":2022,"id":643940,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1675756,"name":"Wenjin Sun","orcid":null,"position":1,"is_corresponding":false},{"id":1015246,"name":"Xiaoshuai Gao","orcid":null,"position":2,"is_corresponding":false},{"id":1013920,"name":"Liao Peng","orcid":"0000-0002-7009-9398","position":3,"is_corresponding":false},{"id":1675757,"name":"Lede Lin","orcid":null,"position":4,"is_corresponding":false},{"id":1675758,"name":"Kaiwen Xiao","orcid":null,"position":5,"is_corresponding":false},{"id":511400,"name":"Yu Liu","orcid":"0000-0002-4789-0288","position":6,"is_corresponding":false},{"id":1013919,"name":"Xingpeng Di","orcid":"0000-0002-1921-5509","position":7,"is_corresponding":false},{"id":1675759,"name":"Shiyu Zhu","orcid":null,"position":8,"is_corresponding":false},{"id":591898,"name":"Huiling Chen","orcid":"0000-0002-7714-9693","position":9,"is_corresponding":false},{"id":452883,"name":"Liang Zhou","orcid":"0000-0003-0820-1520","position":10,"is_corresponding":false},{"id":542963,"name":"Wei Wang","orcid":"0000-0001-8676-1190","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The preventive effects of colony‐stimulating factor 1 receptor (CSF‐1R) inhibition on bladder outlet obstruction induced remodeling","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Introduction</jats:title><jats:p>Bladder outlet obstruction (BOO) is a common problem that can affect bladder structure and function. Currently, there is no effective drugs available to prevent BOO‐induced remodeling. Previous reports have demonstrated that the pathogenesis of BOO is associated with macrophage infiltration and polarization, which is physiologically dependent on colony‐stimulating factor 1 receptor (CSF‐1R) activation. Here we utilized a highly selective CSF‐1R inhibitor, GW2580, to determine its preventive effects on BOO‐induced remodeling.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A total of 24 Sprague–Dawley rats were randomly divided into sham, BOO + vehicle, and BOO + GW2580 group. GW2580 or vehicle control was administrated by oral gavage at daily doses of 40 mg/kg for 6 weeks. Bladder samples were collected for histopathology, immunohistochemistry, immunofluorescence, western blotting, and flow cytometry analysis.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Our results demonstrated that bladder fibrosis was ameliorated by GW2580 compared with the vehicle group (22.01% ± 5.13% vs. 32.15% ± 7.24%, <jats:italic>p</jats:italic> &lt; 0.01). Furthermore, treatment with GW2580 induced an inhibition of macrophage infiltration (4.41% ± 1.28% vs. 13.57% ± 3.42%, <jats:italic>p</jats:italic> &lt; 0.001) and M2 macrophage polarization (10.67% ± 4.15% vs. 28.59% ± 6.38%, <jats:italic>p</jats:italic> &lt; 0.001). There was also a decrease of profibrotic F4/80<jats:sup>+</jats:sup> α‐smooth muscle actin<jats:sup>+</jats:sup> (α‐SMA<jats:sup>+</jats:sup>) macrophage to myofibroblast transition (9.11% ± 2.58% vs. 17.33% ± 4.01%, <jats:italic>p</jats:italic> &lt; 0.001) and CD163<jats:sup>+</jats:sup>TGF‐β1<jats:sup>+</jats:sup> cells (7.68% ± 2.10% vs. 14.17% ± 4.09%, <jats:italic>p</jats:italic> &lt; 0.01) in the GW2580 group when compared with the vehicle group.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In summary, our findings showed that GW2580 is a worthwhile candidate for a follow‐up study to test in the treatment of BOO‐induced remodeling.</jats:p></jats:sec>","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35170790","pmcid":null,"openalex_id":"https://openalex.org/W4213208422","authors":[],"funders":[{"funder_name":"Innovation Spark Project of Sichuan University","grant_id":"","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"","title":null},{"funder_name":"Innovation Spark Project of Sichuan University","grant_id":"","title":null}],"total_grants":4,"fwci":1.9259,"citation_percentile":0.84437513,"influential_citations":0,"citation_trend":[{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/nau.24896","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/nau.24896","host_type":"publisher"},{"url":"https://doi.org/10.1002/nau.24896","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35170790","host_type":"repository"}],"fields_of_study":["Urinary Bladder and Prostate Research","Bladder and Urothelial Cancer Treatments","Tissue Engineering and Regenerative Medicine","Animals","Disease Models, Animal","Female","Follow-Up Studies","Humans","Macrophage Colony-Stimulating Factor","Male","Rats","Rats, Sprague-Dawley","Urinary Bladder","Urinary Bladder Neck Obstruction"],"mesh_terms":["Animals","Urinary Bladder","Urinary Bladder Neck Obstruction","Disease Models, Animal","Female","Follow-Up Studies","Humans","Male","Macrophage Colony-Stimulating Factor","Rats, Sprague-Dawley","Rats"],"keywords":["Medicine","CD163","Bladder outlet obstruction","Fibrosis","CD68","Histopathology","Internal medicine","Myofibroblast","Receptor","Macrophage polarization","M2 Macrophage","Pathogenesis","Immunohistochemistry","Endocrinology","Pathology","Macrophage","Chemistry","Prostate","Csf-1r"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-08T19:54:31.761352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}