{"doi":"10.1002/mrd.70055","title":"Successful Generation of Germline Tyrosinase Gene Edited Thirteen‐Lined Ground Squirrels (<i>Ictidomys tridecemlineatus</i> Mitchill 1821)","abstract":"Thirteen-lined ground squirrels (Ictidomys tridecemlineatus Mitchill 1821; 13-LGS) are useful diurnal rodent models of human cone-mediated vision due to their cone photoreceptor-dominant retinas. To develop the 13-LGS as a better model of inherited human visual disorders, we report a gene-editing protocol targeting the 13-LGS tyrosinase (Tyr) gene. CRISPR/Cas9 microinjection into donor embryos, followed by transfer to pseudo-pregnant recipients, yielded two Tyr-mutated founders. Mating these two to wild-type 13-LGS resulted in 22 offspring, of which five were genotyped with either a 17-bp deletion, 1-bp insertion, or 7-bp deletion Tyr mutation. These results demonstrated that this valuable mammalian model is amenable to germline gene editing by conventional methods.","journal":"Molecular Reproduction and Development","year":2025,"id":574005,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9498,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1101078,"name":"Jamie Foeckler","orcid":null,"position":1,"is_corresponding":false},{"id":1481097,"name":"Emily Knapp","orcid":"0009-0005-3092-5682","position":2,"is_corresponding":false},{"id":471401,"name":"Michael Grzybowski","orcid":"0000-0002-8115-2748","position":3,"is_corresponding":false},{"id":286498,"name":"Aron M. Geurts","orcid":"0000-0002-4347-2505","position":4,"is_corresponding":false},{"id":335079,"name":"Joseph Carroll","orcid":"0000-0002-8640-1029","position":5,"is_corresponding":false},{"id":768165,"name":"Dana K. Merriman","orcid":"0000-0003-2780-0772","position":6,"is_corresponding":false},{"id":1002446,"name":"Akiko Takizawa","orcid":"0000-0001-9101-5512","position":0,"is_corresponding":true}],"reference_count":5,"raw_metadata":null,"created_at":"2026-07-19T02:57:36.753600Z","pmid":"40891659","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}