{"doi":"10.1002/mrd.1120050107","title":"Fertilization of cumulus‐free, zona‐intact mouse ova in vitro at high and low sperm concentrations","abstract":"<jats:title>Abstract</jats:title><jats:p>The effect of varying the sperm concentration between 2 × 10<jats:sup>5</jats:sup> sperm/ml and 8 × 10<jats:sup>6</jats:sup> sperm/ml on fertilization of cumulus‐free, zona‐intact F1 (CBA × C57BL) mouse ova by QS and F1 (CBA × C57BL) mouse spermatozoa was studied. The spermatozoa from both strains of mice exhibited optimal fertilization rates at 2 × 106 sperm/ml. However, at sperm concentrations greater than 4 × 106 sperm/ml and less than 1 × 106 sperm/ml, fertilization rates were significantly reduced. F1 spermatozoa were more susceptible to dilution than QS spermatozoa. A significant interaction between strain and sperm concentration indicated that the two strains produced different fertilization rates at different sperm densities.</jats:p><jats:p>Extracts of epididymal fluid, medium from capacitated spermatozoa, or ampulla fluid did not improve the fertilization rate at 2 × 105 sperm/ml, but retaining the cumulus oophorus did. The decrease in fertilization rate at 8 × 106 sperm/ml can in part be attributed to a nondialysable inhibitor from the neat sperm preparation that appeared to be of epididymal origin.</jats:p>","journal":"Gamete Research","year":1982,"id":13608,"datarank":2.8503543835922374,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":2.355978853691588,"self_endowment_contribution":0.4943755299006494,"citer_contribution":2.355978853691588,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":21,"citers_with_citation_signal":20,"citers_with_endowment":20,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":110931,"name":"P. Quinn","orcid":null,"position":1,"is_corresponding":false},{"id":110930,"name":"J. D. Stanger","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18940862","pmcid":null,"openalex_id":"https://openalex.org/W2079018959","authors":[],"funders":[],"total_grants":0,"fwci":5.3027,"citation_percentile":0.9470292,"influential_citations":1,"citation_trend":[{"year":2012,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.1120050107","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.1120050107","host_type":"publisher"},{"url":"https://doi.org/10.1002/mrd.1120050107","host_type":"journal"},{"url":"https://mouseion.jax.org/ssbb1982/2785","host_type":"repository"}],"fields_of_study":["Sperm and Testicular Function","Reproductive Biology and Fertility","Ovarian function and disorders","Biology"],"mesh_terms":[],"keywords":["Sperm","Human fertilization","Andrology","Biology","Cumulus oophorus","Semen","Fertilisation","Zona pellucida","Sperm motility","Oocyte","Anatomy","Cryopreservation","Embryo","Botany","Genetics","Reproductive technology","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-05-31T05:16:21.051397Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}