{"doi":"10.1002/mnfr.202300134","title":"Alanine Scanning of the Unstructured Region of Ara h 2 and of a Related Mimotope Reveals Critical Amino Acids for IgE Binding","abstract":"SCOPE: The unstructured region of Ara h 2, referred to as epitope 3, contains a repeated motif, DYPSh (h = hydroxyproline) that is important for IgE binding. METHODS AND RESULTS: IgE binding assays to 20mer and shorter peptides of epitope 3, defines a 16mer core sequence containing one copy of the DPYSh motif, DEDSYERDPYShSQDP. This study performs alanine scanning of this and a related 12mer mimotope, LLDPYAhRAWTK. IgE binding, using a pool of 10 sera and with individual sera, is greatly reduced when alanine is substituted for aspartate at position 8 (D8; p < 0.01), tyrosine at position 10 (Y10; p < 0.01), and hydroxyproline at position 12 (h12; p < 0.001). IgE binding to alanine-substituted peptides of a mimotope containing the DPY_h motif confirm the critical importance of Y (p < 0.01) and h (p < 0.01), but not D. Molecular modeling of the core and mimotope suggests an h-dependent conformational basis for the recognition of these sequences by polyclonal IgE. CONCLUSIONS: IgE from pooled sera and individual sera differentially bound amino acids throughout the sequences of Epitope 3 and its mimotope, with Y10 and h12 being most important for all sera. These results are highly significant for designing hypoallergenic forms of Ara h 2.","journal":"Molecular Nutrition & Food Research","year":2023,"id":351679,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9424,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":294062,"name":"Catherine H. Schein","orcid":"0000-0002-8290-2109","position":1,"is_corresponding":false},{"id":294060,"name":"Werner Braun","orcid":"0000-0002-7581-168X","position":2,"is_corresponding":false},{"id":296462,"name":"Surendra S. Negi","orcid":null,"position":3,"is_corresponding":false},{"id":294061,"name":"Xueni Chen","orcid":"0000-0002-5181-4536","position":4,"is_corresponding":false},{"id":370702,"name":"Michael D. Kulis","orcid":"0000-0001-8092-7444","position":5,"is_corresponding":false},{"id":294394,"name":"Edwin Kim","orcid":"0000-0001-6418-1785","position":6,"is_corresponding":false},{"id":1097265,"name":"Vidya Pathy","orcid":null,"position":7,"is_corresponding":false},{"id":963798,"name":"Marina Pozzoli","orcid":null,"position":8,"is_corresponding":false},{"id":587106,"name":"Weimin Liu","orcid":"0000-0001-7507-6032","position":9,"is_corresponding":false},{"id":294058,"name":"Stephen C. Dreskin","orcid":"0000-0003-3548-5530","position":10,"is_corresponding":false},{"id":1097264,"name":"Nicole Canon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T01:12:45.897709Z","pmid":"37706599","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}