{"doi":"10.1002/mnfr.201901166","title":"Consumption of Diet Soda Sweetened with Sucralose and Acesulfame‐Potassium Alters Inflammatory Transcriptome Pathways in Females with Overweight and Obesity","abstract":"SCOPE: Low-calorie sweetener (LCS) consumption is associated with metabolic disease in observational studies. However, physiologic mechanisms underlying LCS-induced metabolic impairments in humans are unclear. This study is aimed at identifying molecular pathways in adipose impacted by LCSs. METHODS AND RESULTS: Seven females with overweight or obesity, who did not report LCS use, consumed 12 ounces of diet soda containing sucralose and acesulfame-potassium (Ace-K) three times daily for 8 weeks. A subcutaneous adipose biopsy from the left abdomen and a fasting blood sample were collected at baseline and post-intervention. Global gene expression were assessed using RNA-sequencing followed by functional pathway analysis. No differences in circulating metabolic or inflammatory biomarkers were observed. However, ANOVA detected 828 differentially expressed annotated genes after diet soda consumption (p < 0.05), including transcripts for inflammatory cytokines. Fifty-eight of 140 canonical pathways represented in pathway analyses regulated inflammation, and several key upstream regulators of inflammation (e.g., TNF-alpha) were also represented. CONCLUSION: Consumption of diet soda with sucralose and Ace-K alters inflammatory transcriptomic pathways (e.g., NF-κB signaling) in subcutaneous adipose tissue but does not significantly alter circulating biomarkers. Findings highlight the need to examine molecular and metabolic effects of LCS exposure in a larger randomized control trial for a longer duration.","journal":"Molecular Nutrition & Food Research","year":2020,"id":96424,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.7301,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":477759,"name":"Sabyasachi Sen","orcid":"0000-0002-3675-9129","position":1,"is_corresponding":false},{"id":478382,"name":"Patrick Merkel","orcid":null,"position":2,"is_corresponding":false},{"id":477760,"name":"Fiona J. Dore","orcid":"0000-0003-2369-5717","position":3,"is_corresponding":false},{"id":477761,"name":"David Stern","orcid":"0000-0003-3363-6040","position":4,"is_corresponding":false},{"id":465144,"name":"Curtis J. Henry","orcid":"0000-0002-5523-554X","position":5,"is_corresponding":false},{"id":231167,"name":"Hongyi Cai","orcid":"0000-0002-8021-8493","position":6,"is_corresponding":false},{"id":231170,"name":"Peter J. Walter","orcid":"0000-0001-8571-398X","position":7,"is_corresponding":false},{"id":6545,"name":"Keith A. Crandall","orcid":"0000-0002-0836-3389","position":8,"is_corresponding":false},{"id":446035,"name":"Kristina I. Rother","orcid":"0000-0002-8421-131X","position":9,"is_corresponding":false},{"id":477762,"name":"Monica J. Hubal","orcid":"0000-0001-6579-6036","position":10,"is_corresponding":false},{"id":361593,"name":"Allison C. Sylvetsky","orcid":"0000-0003-0424-2798","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T22:34:34.781847Z","pmid":"32281732","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}