{"doi":"10.1002/mds.29798","title":"Alterations of Peripheral Lymphocyte Subsets in Isolated Rapid Eye Movement Sleep Behavior Disorder","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Adaptive immune dysfunction may play a crucial role in Parkinson's disease (PD) development. Isolated rapid eye movement sleep behavior disorder (iRBD) represents the prodromal stage of synucleinopathies, including PD. Elucidating the peripheral adaptive immune system is crucial in iRBD, but current knowledge remains limited.</jats:p></jats:sec><jats:sec><jats:title>Objective</jats:title><jats:p>This study aimed to characterize peripheral lymphocyte profiles in iRBD patients compared with healthy control subjects (HCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>This cross‐sectional study recruited polysomnography‐confirmed iRBD patients and age‐ and sex‐matched HCs. Venous blood was collected from each participant. Flow cytometry was used to evaluate surface markers and intracellular cytokine production in peripheral blood mononuclear cells.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Forty‐four iRBD patients and 36 HCs were included. Compared with HCs, patients with iRBD exhibited significant decreases in absolute counts of total lymphocytes and CD3<jats:sup>+</jats:sup> T cells. In terms of T cell subsets, iRBD patients showed higher frequencies and counts of proinflammatory T helper 1 cells and INF‐γ<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, along with lower frequencies and counts of anti‐inflammatory T helper 2 cells. A significant increase in the frequency of central memory T cells in CD8<jats:sup>+</jats:sup> T cells was also observed in iRBD. Regarding B cells, iRBD patients demonstrated reduced frequencies and counts of double‐negative memory B cells compared with control subjects.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>This study demonstrated alterations in the peripheral adaptive immune system in iRBD, specifically in CD4<jats:sup>+</jats:sup> and INF‐γ<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cell subsets. An overall shift toward a proinflammatory state of adaptive immunity was already evident in iRBD. These observations might provide insights into the optimal timing for initiating immune interventions in PD. © 2024 The Authors. <jats:italic>Movement Disorders</jats:italic> published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.</jats:p></jats:sec>","journal":"Movement Disorders","year":2024,"id":648291,"datarank":0.40845576866673244,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.035719771198532306,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.035719771198532306,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":10,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":428178,"name":"Yatong Li","orcid":"0000-0003-2021-4076","position":1,"is_corresponding":false},{"id":1689402,"name":"Huihui Cai","orcid":null,"position":2,"is_corresponding":false},{"id":1689403,"name":"Wenyi Kou","orcid":null,"position":3,"is_corresponding":false},{"id":628579,"name":"Chen Yang","orcid":"0000-0001-6011-4067","position":4,"is_corresponding":false},{"id":302835,"name":"Siming Li","orcid":"0000-0001-7077-0404","position":5,"is_corresponding":false},{"id":388075,"name":"Jiawei Wang","orcid":"0000-0001-9893-8539","position":6,"is_corresponding":false},{"id":921484,"name":"Ning Zhang","orcid":"0000-0003-2095-5261","position":7,"is_corresponding":false},{"id":277420,"name":"Tao Feng","orcid":"0000-0002-8530-4622","position":8,"is_corresponding":false},{"id":1572637,"name":"Yuanchu Zheng","orcid":"0000-0002-9080-6694","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Alterations of Peripheral Lymphocyte Subsets in Isolated Rapid Eye Movement Sleep Behavior Disorder","abstract":"<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Adaptive immune dysfunction may play a crucial role in Parkinson's disease (PD) development. Isolated rapid eye movement sleep behavior disorder (iRBD) represents the prodromal stage of synucleinopathies, including PD. Elucidating the peripheral adaptive immune system is crucial in iRBD, but current knowledge remains limited.</jats:p></jats:sec><jats:sec><jats:title>Objective</jats:title><jats:p>This study aimed to characterize peripheral lymphocyte profiles in iRBD patients compared with healthy control subjects (HCs).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>This cross‐sectional study recruited polysomnography‐confirmed iRBD patients and age‐ and sex‐matched HCs. Venous blood was collected from each participant. Flow cytometry was used to evaluate surface markers and intracellular cytokine production in peripheral blood mononuclear cells.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Forty‐four iRBD patients and 36 HCs were included. Compared with HCs, patients with iRBD exhibited significant decreases in absolute counts of total lymphocytes and CD3<jats:sup>+</jats:sup> T cells. In terms of T cell subsets, iRBD patients showed higher frequencies and counts of proinflammatory T helper 1 cells and INF‐γ<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, along with lower frequencies and counts of anti‐inflammatory T helper 2 cells. A significant increase in the frequency of central memory T cells in CD8<jats:sup>+</jats:sup> T cells was also observed in iRBD. Regarding B cells, iRBD patients demonstrated reduced frequencies and counts of double‐negative memory B cells compared with control subjects.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>This study demonstrated alterations in the peripheral adaptive immune system in iRBD, specifically in CD4<jats:sup>+</jats:sup> and INF‐γ<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cell subsets. An overall shift toward a proinflammatory state of adaptive immunity was already evident in iRBD. These observations might provide insights into the optimal timing for initiating immune interventions in PD. © 2024 The Authors. <jats:italic>Movement Disorders</jats:italic> published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.</jats:p></jats:sec>","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38529776","pmcid":null,"openalex_id":"https://openalex.org/W4393193625","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"82071422","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82271459","title":null},{"funder_name":"Beijing Municipal Natural Science Foundation","grant_id":"7212031","title":null},{"funder_name":"Beijing Municipal Natural Science Foundation","grant_id":"7244331","title":null}],"total_grants":4,"fwci":2.7436,"citation_percentile":0.90733641,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":7}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mds.29798","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mds.29798","host_type":"publisher"},{"url":"https://movementdisorders.onlinelibrary.wiley.com/doi/pdf/10.1002/mds.29798","host_type":"publisher"},{"url":"https://doi.org/10.1002/mds.29798","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38529776","host_type":"repository"}],"fields_of_study":["Parkinson's Disease Mechanisms and Treatments","Sleep and Wakefulness Research","Restless Legs Syndrome Research","Humans","Male","REM Sleep Behavior Disorder","Female","Aged","Middle Aged","Cross-Sectional Studies","Lymphocyte Subsets","Parkinson Disease","Polysomnography"],"mesh_terms":["Aged","Cross-Sectional Studies","Female","Humans","Male","Middle Aged","Parkinson Disease","Lymphocyte Subsets","Polysomnography","REM Sleep Behavior Disorder"],"keywords":["Immune system","Immunology","CD8","Rapid eye movement sleep","T cell","Medicine","Acquired immune system","Proinflammatory cytokine","Lymphocyte","Biology","Neuroscience","Inflammation","Eye movement","Lymphocyte subsets","Adaptive Immune System","Irbd","Proinflammatory State"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T02:27:48.625517Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}