{"doi":"10.1002/jrs.675","title":"Raman spectroscopic characterization of <i>Bombyx mori</i> silk fibroin: Raman spectrum of Silk I","abstract":"<jats:title>Abstract</jats:title><jats:p>This study focuses on the conformational characterization of differently processed <jats:italic>Bombyx mori</jats:italic> silk fibroin samples by Raman spectroscopy. The Raman spectra of silk fibroin film and liquid silk are discussed in comparison with those of the crystalline fractions of <jats:italic>Bombyx mori</jats:italic> silk fibroin (Cp, chymotryptic precipitate) with Silk I (Silk I‐Cp) and Silk II (Silk II‐Cp) structures. The complete 1800–200 cm<jats:sup>−1</jats:sup> Raman spectrum of Silk I‐Cp is reported for the first time. The amide I and amide III modes were found to be scarcely suitable for the spectroscopic characterization of silk fibroin in the Silk I form in the presence of a random coil conformation. Raman marker bands for the Silk I form were identified in other spectral ranges at about 1415, 950, 930, 865, 260 and 230 cm<jats:sup>−1</jats:sup>. On the basis of the above findings, the comparison of the Raman spectra of film, liquid silk and Silk I‐Cp in the range 1000–800 cm<jats:sup>−1</jats:sup> clearly indicates that in addition to random coil, both film and liquid silk contain local domains of Silk I structure; their amount is higher in liquid silk, as indicated by the relative intensity of the bands at about 950, 930 and 865 cm<jats:sup>−1</jats:sup> and by the <jats:italic>I</jats:italic><jats:sub>1415</jats:sub>/<jats:italic>I</jats:italic><jats:sub>1455</jats:sub> intensity ratio.</jats:p><jats:p>The assignments of the bands at about 1275 and 1107 cm<jats:sup>−1</jats:sup> are also discussed. These bands were previously assigned to the presence of α‐helical conformation in <jats:italic>Bombyx mori</jats:italic> silk but, from the results reported, they should rather be attributed to the Silk I form. Copyright © 2001 John Wiley &amp; Sons, Ltd.</jats:p>","journal":"Journal of Raman Spectroscopy","year":2001,"id":22120,"datarank":9.125212322667611,"base_score":5.056245805348308,"endowment":5.056245805348308,"self_citation_contribution":0.7584368708022463,"citation_network_contribution":8.366775451865365,"self_endowment_contribution":0.7584368708022463,"citer_contribution":8.366775451865365,"corpus_percentile":null,"corpus_rank":null,"citation_count":156,"citer_count":137,"citers_with_citation_signal":127,"citers_with_endowment":127,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":140145,"name":"P. Taddei","orcid":null,"position":1,"is_corresponding":false},{"id":140146,"name":"G. Freddi","orcid":null,"position":2,"is_corresponding":false},{"id":140147,"name":"T. Asakura","orcid":null,"position":3,"is_corresponding":false},{"id":140148,"name":"M. Tsukada","orcid":null,"position":4,"is_corresponding":false},{"id":140144,"name":"P. Monti","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.056245805348308,"endowment":5.056245805348308,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W2053642165","authors":[],"funders":[],"total_grants":0,"fwci":4.3105,"citation_percentile":0.94254446,"influential_citations":6,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":9},{"year":2014,"count":9},{"year":2015,"count":5},{"year":2016,"count":10},{"year":2017,"count":5},{"year":2018,"count":6},{"year":2019,"count":8},{"year":2020,"count":10},{"year":2021,"count":10},{"year":2022,"count":4},{"year":2023,"count":6},{"year":2024,"count":10},{"year":2025,"count":8},{"year":2026,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjrs.675","host_type":"publisher"},{"url":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/jrs.675","host_type":"publisher"},{"url":"https://doi.org/10.1002/jrs.675","host_type":"journal"}],"fields_of_study":["Silk-based biomaterials and applications","Silkworms and Sericulture Research","Neurobiology and Insect Physiology Research","Chemistry","Materials Science"],"mesh_terms":[],"keywords":["Fibroin","SILK","Bombyx mori","Raman spectroscopy","Random coil","Materials science","Chemistry","Polymer chemistry","Crystallography","Composite material","Circular dichroism","Optics","Biochemistry","Physics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T17:58:58.678188Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}