{"doi":"10.1002/jobm.70006","title":"Cultivation Strategies to Improve <i>Chlamydomonas reinhardtii</i> Growth and Recombinant Mcherry Expression","abstract":"<jats:title>ABSTRACT</jats:title><jats:p><jats:italic>Chlamydomonas reinhardtii</jats:italic> is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH<jats:sub>4</jats:sub>Cl) concentration and temperature on the growth of transgenic <jats:italic>C. reinhardtii</jats:italic> expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH<jats:sub>4</jats:sub>Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25°C and 32.1°C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15°C–17°C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect <jats:italic>C. reinhardtii</jats:italic> growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in <jats:italic>C. reinhardtii</jats:italic>. These findings pave the way for scaling up the use of <jats:italic>C. reinhardtii</jats:italic> as a biofactory in industry and can be applied to other microalgal systems.</jats:p>","journal":"Journal of Basic Microbiology","year":2025,"id":682431,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1782854,"name":"Bruno Guzzo da Silva","orcid":null,"position":1,"is_corresponding":false},{"id":1782855,"name":"Ednilson Donisete de França Júnior","orcid":null,"position":2,"is_corresponding":false},{"id":1782856,"name":"Isac José da Silva Filho","orcid":null,"position":3,"is_corresponding":false},{"id":1375778,"name":"João Vitor Dutra Molino","orcid":"0000-0003-2475-9807","position":4,"is_corresponding":false},{"id":1782857,"name":"João Carlos Monteiro de Carvalho","orcid":null,"position":5,"is_corresponding":false},{"id":1782858,"name":"Livia Seno Ferreira‐Camargo","orcid":null,"position":6,"is_corresponding":false},{"id":1782853,"name":"Jassiara da Silva Pessoa","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cultivation Strategies to Improve <i>Chlamydomonas reinhardtii</i> Growth and Recombinant Mcherry Expression","abstract":"<jats:title>ABSTRACT</jats:title><jats:p><jats:italic>Chlamydomonas reinhardtii</jats:italic> is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH<jats:sub>4</jats:sub>Cl) concentration and temperature on the growth of transgenic <jats:italic>C. reinhardtii</jats:italic> expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH<jats:sub>4</jats:sub>Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25°C and 32.1°C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15°C–17°C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect <jats:italic>C. reinhardtii</jats:italic> growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in <jats:italic>C. reinhardtii</jats:italic>. These findings pave the way for scaling up the use of <jats:italic>C. reinhardtii</jats:italic> as a biofactory in industry and can be applied to other microalgal systems.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39936603","pmcid":null,"openalex_id":"https://openalex.org/W4407409693","authors":[],"funders":[{"funder_name":"This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Processes no 2019/13822-5 and 2016/12992-6).","grant_id":"","title":null}],"total_grants":1,"fwci":0.4507,"citation_percentile":0.55109461,"influential_citations":0,"citation_trend":[{"year":2025,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.70006","host_type":"publisher"},{"url":"https://doi.org/10.1002/jobm.70006","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39936603","host_type":"repository"}],"fields_of_study":["Algal biology and biofuel production","Photosynthetic Processes and Mechanisms","Micro and Nano Robotics","Medicine","Environmental Science","Biology","Engineering","Materials Science"],"mesh_terms":["Red Fluorescent Protein","Ammonium Chloride","Culture Media","Luminescent Proteins","Recombinant Proteins","Temperature","Gene Expression","Chlamydomonas reinhardtii"],"keywords":["Chlamydomonas reinhardtii","mCherry","Recombinant DNA","Green fluorescent protein","In silico","Transgene","Chemistry","Ammonium sulfate","Yield (engineering)","Biochemistry","Cell biology","Biology","Biophysics","Gene","Mutant","Chromatography","Materials science","Fluorescent Protein","Ccrd","Growth Optimization","Transgenic Microalga"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T20:21:58.720249Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}