{"doi":"10.1002/jobm.201800160","title":"Establishment of a low‐dosage‐IPTG inducible expression system construction method in <i>Escherichia coli</i>","abstract":"<jats:sec><jats:label/><jats:p>The <jats:italic>lac</jats:italic> operon is a delicate inducible gene expression element in bacteria. To efficiently induce gene expression, a sufficient dosage of an inducer, usually that of 500–1000 µM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG), is required to keep repressor LacI from its binding sites, which is a heavy cost burden in low‐value‐added products. So we propose a strategy to reduce the required dosage of IPTG by restricting LacI expression. To test this strategy, we employed a reconstructed IPTG inducible expression system based on <jats:italic>lac</jats:italic> operon, Promoter(<jats:italic>lacO</jats:italic>)‐target gene‐P<jats:italic>tac</jats:italic>L‐<jats:italic>lacI</jats:italic>, where a modified promoter, P<jats:italic>tac</jats:italic>, with a random synthetic library (P<jats:italic>tac</jats:italic>L) to instead of P<jats:italic>lacI</jats:italic> to optimize LacI expression in <jats:italic>Escherichia coli</jats:italic>. Finally, the P<jats:italic>tac</jats:italic>L mutant, P<jats:italic>tac</jats:italic>L4, which could maintain the same repression effect as the original P<jats:italic>lacI</jats:italic> while reducing the required dosage of IPTG from 500 to 20 µM, was selected. This method is simple and efficient and can be of a good reference point for attempts to reduce inducer concentration in the IPTG or similar inducible expression systems.</jats:p></jats:sec>","journal":"Journal of Basic Microbiology","year":2018,"id":40481,"datarank":0.8711979128532092,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.47533931341092034,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.47533931341092034,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":12,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":196607,"name":"Xin‐Yi Tao","orcid":null,"position":1,"is_corresponding":false},{"id":196608,"name":"Feng‐Qing Wang","orcid":"0000-0002-3473-5991","position":2,"is_corresponding":false},{"id":196609,"name":"Yu‐Hong Ren","orcid":null,"position":3,"is_corresponding":false},{"id":196610,"name":"Dong‐Zhi Wei","orcid":null,"position":4,"is_corresponding":false},{"id":169652,"name":"Ming Zhao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29962051","pmcid":null,"openalex_id":"https://openalex.org/W2811441309","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"21776075","title":null}],"total_grants":1,"fwci":0.7167,"citation_percentile":0.75743666,"influential_citations":1,"citation_trend":[{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201800160","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201800160","host_type":"publisher"},{"url":"https://doi.org/10.1002/jobm.201800160","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29962051","host_type":"repository"}],"fields_of_study":["Bacterial Genetics and Biotechnology","Bacteriophages and microbial interactions","RNA and protein synthesis mechanisms","Medicine","Chemistry","Engineering","Biology","Binding Sites","Escherichia coli","Escherichia coli Proteins","Gene Expression","Genetic Techniques","Isopropyl Thiogalactoside","Lac Operon","Lac Repressors","Plasmids","Promoter Regions, Genetic","Small Molecule Libraries"],"mesh_terms":["Binding Sites","Escherichia coli","Genetic Techniques","Isopropyl Thiogalactoside","Lac Operon","Plasmids","Promoter Regions, Genetic","Gene Expression","Escherichia coli Proteins","Small Molecule Libraries","Lac Repressors"],"keywords":["Lac repressor","lac operon","Inducer","Escherichia coli","Operon","Repressor","Chemistry","Molecular biology","Mutant","Gene","Promoter","Gene expression","Beta-galactosidase","Biology","Biochemistry","Gene expression system","Synthetic Promoter Library","Low-dosage-iptg"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-12T06:34:54.095016Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}