{"doi":"10.1002/jmv.29133","title":"Follow‐up of humoral immune response after HPV vaccination using first‐void urine: A longitudinal cohort study","abstract":"Assessment of humoral immune responses following human papillomavirus (HPV) vaccination currently relies on invasive blood sampling. This longitudinal cohort study explores the usability of first-void urine as a noninvasive alternative sample for antibody detection. In this study, 58 women receiving three doses of the 9vHPV vaccine within a Gardasil9 (9vHPV) Phase III randomized controlled trial were included. Participants provided paired first-void urine and blood samples before vaccination (M0), 1 month after the third dose (M7), and ~3 years after the third dose (M43). Type-specific antibody responses to the 9vHPV types were analyzed in 174 first-void urine and 172 serum samples using a virus-like particle-based IgG multiplex enzyme-linked immunosorbent assay. Additionally, total human IgG concentrations were determined using the BioPlex assay. At M7, 1 month after complete 9vHPV vaccination, 95%-100% of first-void urine and 100% of serum samples had detectable concentrations, varying by HPV type. At M43, 84%-100% of first-void urine and 98%-100% of serum samples had HPV-specific antibody concentrations. Results show significant Spearman rank correlations between type-specific HPV-antibody concentrations for paired first-void urine and serum at all time points. This study confirms the potential feasibility of utilizing first-void urine as a noninvasive immunological sample within HPV vaccine trials.","journal":"Journal of Medical Virology","year":2023,"id":346845,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9392,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1087735,"name":"Jade Pattyn","orcid":"0000-0002-4538-9027","position":1,"is_corresponding":false},{"id":1087736,"name":"Severien Van Keer","orcid":"0000-0003-1842-7478","position":2,"is_corresponding":false},{"id":1088257,"name":"Annemie De Smet","orcid":null,"position":3,"is_corresponding":false},{"id":242949,"name":"Ilse De Coster","orcid":"0000-0002-4847-2328","position":4,"is_corresponding":false},{"id":1087737,"name":"Wiebren Tjalma","orcid":"0000-0002-6618-045X","position":5,"is_corresponding":false},{"id":748964,"name":"Ira Rajbhandari","orcid":null,"position":6,"is_corresponding":false},{"id":472574,"name":"Gitika Panicker","orcid":null,"position":7,"is_corresponding":false},{"id":355575,"name":"Elizabeth R. Unger","orcid":"0000-0002-2925-5635","position":8,"is_corresponding":false},{"id":1087738,"name":"Alex Vorsters","orcid":"0000-0002-0265-058X","position":9,"is_corresponding":false},{"id":1087734,"name":"Laura Téblick","orcid":"0000-0002-7484-0956","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T01:11:53.085356Z","pmid":"37812015","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}