{"doi":"10.1002/jmv.28472","title":"Microglia innate immune response contributes to the antiviral defense and blood–CSF barrier function in human choroid plexus organoids during HSV‐1 infection","abstract":"The choroid plexus (ChP) is the source of cerebrospinal fluid (CSF). The ChP-CSF system not only provides the necessary cushion for the brain but also works as a sink for waste clearance. During sepsis, pathogens and host immune cells can weaken the ChP barrier and enter the brain, causing cerebral dysfunctions known as sepsis-associated encephalophagy. Here, we used human ChP organoid (ChPO) to model herpes simplex virus type 1 (HSV-1) infection and found ChP epithelial cells were highly susceptible to HSV-1. Since the current ChPO model lacks a functional innate immune component, particularly microglia, we next developed a new microglia-containing ChPO model, and found microglia could effectively limit HSV-1 infection and protect epithelial barrier in ChPOs. Furthermore, we found the innate immune cyclic GMP-AMP synthase (cGAS)-STING pathway and its downstream interferon response were essential, as cGAS inhibitor RU.512 or STING inhibitor H-151 abolished microglia antiviral function and worsened ChP barrier in organoids. These results together indicated that cGAS-STING pathway coordinates antiviral response in ChP and contributes to treating sepsis or related neurological conditions.","journal":"Journal of Medical Virology","year":2023,"id":331590,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9567,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1057624,"name":"Yuanpu Chiu","orcid":null,"position":1,"is_corresponding":false},{"id":573968,"name":"Xinyan Liang","orcid":"0000-0003-3275-8548","position":2,"is_corresponding":false},{"id":289058,"name":"Shangzhou Xia","orcid":"0000-0001-9871-739X","position":3,"is_corresponding":false},{"id":1057625,"name":"Mariam Ayrapetyan","orcid":null,"position":4,"is_corresponding":false},{"id":1009892,"name":"Siqi Liu","orcid":"0000-0002-2767-641X","position":5,"is_corresponding":false},{"id":1057626,"name":"Cuiling He","orcid":null,"position":6,"is_corresponding":false},{"id":1057627,"name":"Ruocen Song","orcid":null,"position":7,"is_corresponding":false},{"id":304615,"name":"Jianxiong Zeng","orcid":"0000-0002-7339-734X","position":8,"is_corresponding":false},{"id":1030557,"name":"Xiangxue Deng","orcid":"0009-0000-8172-987X","position":9,"is_corresponding":false},{"id":248983,"name":"Weiming Yuan","orcid":"0000-0002-4780-7157","position":10,"is_corresponding":false},{"id":294874,"name":"Zhen Zhao","orcid":"0000-0001-8967-5570","position":11,"is_corresponding":false},{"id":1057623,"name":"Haowen Qiao","orcid":null,"position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T01:09:25.015646Z","pmid":"36606611","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}