{"doi":"10.1002/jlb.42.5.485","title":"Generation of Macrophage Variants With 5-Azacytidine: Selection for Mannose Receptor Expression","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Mannose receptors are expressed only in primary macrophages. Established macrophage-derived cell lines, although apparently possessing the potential to synthesize mannose receptors, do not express them on their plasma membranes. Using the drug 5-Azacytidine, mannose receptor expression was induced in the macrophage-derived mouse cell line J774. Receptor positive cells were sorted through a fluorescent activated cell sorter (FACS) prior to cloning. Clones were isolated which continuously express mannose receptors in culture. These macrophages were able to endocytose β-glucuronidase and phagocytose yeast particles via mannose receptors. Secretion of the lysosomal enzyme β-hexosaminidase was also reduced in proportion to the degree of mannose receptor expression.</jats:p>","journal":"Journal of Leukocyte Biology","year":1987,"id":636770,"datarank":0.62147020895873,"base_score":4.143134726391533,"endowment":4.143134726391533,"self_citation_contribution":0.62147020895873,"citation_network_contribution":0.0,"self_endowment_contribution":0.62147020895873,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1653023,"name":"Melinda S Leech","orcid":null,"position":1,"is_corresponding":false},{"id":1653024,"name":"Philip D Stahl","orcid":null,"position":2,"is_corresponding":false},{"id":1653022,"name":"Stephanie Diment","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Generation of Macrophage Variants With 5-Azacytidine: Selection for Mannose Receptor Expression","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Mannose receptors are expressed only in primary macrophages. Established macrophage-derived cell lines, although apparently possessing the potential to synthesize mannose receptors, do not express them on their plasma membranes. Using the drug 5-Azacytidine, mannose receptor expression was induced in the macrophage-derived mouse cell line J774. Receptor positive cells were sorted through a fluorescent activated cell sorter (FACS) prior to cloning. Clones were isolated which continuously express mannose receptors in culture. These macrophages were able to endocytose β-glucuronidase and phagocytose yeast particles via mannose receptors. Secretion of the lysosomal enzyme β-hexosaminidase was also reduced in proportion to the degree of mannose receptor expression.</jats:p>","is_dataset_classified":null,"base_score":4.143134726391533,"endowment":4.143134726391533,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2445884","pmcid":null,"openalex_id":"https://openalex.org/W2338171589","authors":[],"funders":[],"total_grants":0,"fwci":0.6291,"citation_percentile":0.72190784,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":4},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjlb.42.5.485","host_type":"publisher"},{"url":"https://academic.oup.com/jleukbio/article-pdf/42/5/485/49721324/jlb0485.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1002/jlb.42.5.485","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2445884","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.557.3662","host_type":""}],"fields_of_study":["HIV Research and Treatment","Phagocytosis and Immune Regulation","Transgenic Plants and Applications"],"mesh_terms":["Mannose Receptor","Animals","Azacitidine","Cell Line","Cell Membrane","Clone Cells","Endocytosis","Flow Cytometry","Fluoresceins","Fluorescent Dyes","Gene Expression Regulation","Glucuronidase","Macrophages","Receptors, Cell Surface","Receptors, Immunologic","Thiocyanates","Genetic Variation","Fluorescein-5-isothiocyanate","Lectins, C-Type","Mannose-Binding Lectins","Mice"],"keywords":["Mannose receptor","Mannose","Biology","Receptor","Expression cloning","Macrophage","Cell culture","Cell biology","Molecular biology","Receptor expression","Phagocytosis","Biochemistry","In vitro","Complementary DNA","Gene","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T17:42:32.799820Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}