{"doi":"10.1002/jia2.26496","title":"Dosing forgiveness of oral PrEP for cisgender women remains uncertain","abstract":"Pre-exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate and emtricitabine (TDF/FTC) has been proven safe and effective for preventing HIV acquisition, when taken daily, in men and transgender women who have sex with men (MSM/TGW) and cisgender women (hereafter, women). Based on existing evidence, we can have high confidence that as few as 4 pills per week reduce HIV incidence by at least 90% in MSM/TGW. In addition, a “2-1-1” regimen in which two pills are taken prior to a potential HIV exposure followed by one pill in each of the two following days has been clinically proven to substantially reduce HIV incidence in MSM/TGW. However, the same level of support is not yet available for either dosing forgiveness or the efficacy of “2-1-1” event-driven PrEP in women. One of the most controversial notions in HIV therapeutics is whether women require different adherence or dosing strategies compared with MSM/TGW by virtue of differences in drug distribution between the female genital tract and rectal tissue [1, 2]. The TDF/FTC adherence-efficacy curve has previously been established in MSM/TGW using levels of intraerythrocytic tenofovir-diphosphate (TFV-DP) in incident cases of HIV and matched controls from iPrEx, iPrEx OLE and recently reinforced with HPTN 083 [3-5]. Four secondary analyses have assessed the relationship between TDF/FTC adherence and HIV incidence in women [5-8]. These analyses have prompted renewed discussion on the dosing forgiveness in this population and their potential to benefit from on-demand PrEP [2, 9, 10]. Here, we outline the methodological differences in the latest studies and discuss potential implications for clinical practice guidelines. Of the four analyses, three concluded that women need to adhere to daily dosing to achieve a 90% reduction in HIV incidence (Figure 1). Two subgroup analyses of non-randomized cohorts of PrEP users compared HIV incidence in those with high adherence to low adherence [5, 8]. First, women enrolled in HPTN 084 and MSM/TGW enrolled in HPTN 083 had their adherence assessed with intraerythrocytic TFV-DP, which quantifies adherence over the prior 1–2 months. HIV incidence in those assessed to take <2, 2–3, 4–6 or 7 pills per week was compared to HIV incidence in those with no quantifiable TFV-DP [5]. Although the confidence intervals were wide, this analysis suggested that women need to adhere to daily pills to gain the same benefit from PrEP as MSM/TGW taking 2–3 pills per week. Second, a meta-analysis analysed the adherence of 6296 women enrolled in 11 demonstration projects over 8 years [8]. HIV incidence was calculated in four sub-populations based on adherence which was assessed by various methods including self-report and drug concentrations. The reduction in HIV incidence among those taking 4–6 and 7 pills per week compared to those taking <2 was comparable to that of Anderson et al. Third, a modelling study reanalysed plasma tenofovir data from placebo-controlled efficacy studies to derive a relationship between the frequency of pill taking and PrEP efficacy [6]. Moore et al. imputed intraerythrocytic TFV-DP concentrations from plasma TFV quantifiability with data from HPTN 082 (which collected both plasma and TFV-DP in dried blood spots) and then calibrated a dose-efficacy curve to HIV incidence in the placebo and active arms of VOICE, FEM-PrEP and Partners PrEP. Study results suggest that daily pill taking is required for heterosexual women to achieve the same PrEP benefit as MSM/TGW taking 4 pills per week. In contrast, Zhang et al. adapted a pharmacokinetic-pharmacodynamic model of TDF/FTC, previously calibrated to in-vitro antiretroviral assays, to predict PrEP efficacy based on projected concentrations of active drug metabolites in tissue compartments [7]. They tested hypotheses that intracellular concentrations in either peripheral blood mononuclear cells or local tissues completely determine TDF/FTC efficacy across a range of TDF/FTC doses per wee","journal":"Journal of the International AIDS Society","year":2025,"id":541224,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9547,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":316641,"name":"David V. Glidden","orcid":"0000-0001-5888-1419","position":1,"is_corresponding":false},{"id":279022,"name":"Peter L. Anderson","orcid":"0000-0002-1200-8494","position":2,"is_corresponding":false},{"id":366533,"name":"Craig W. Hendrix","orcid":"0000-0002-5696-8665","position":3,"is_corresponding":false},{"id":374735,"name":"Dobromir Dimitrov","orcid":"0000-0002-2842-5436","position":4,"is_corresponding":false},{"id":425962,"name":"Mia Moore","orcid":"0000-0001-5055-7075","position":0,"is_corresponding":true}],"reference_count":12,"raw_metadata":null,"created_at":"2026-07-19T02:52:47.161928Z","pmid":"40384398","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}