{"doi":"10.1002/jev2.12329","title":"ISEV2023 Abstract Book","abstract":"Introduction: Cells actively secrete extracellular vesicles and particles (EVs), transporting selectively packaged functional biomolecules to mediate intercellular communication in normal physiology and pathology. We previously showed that EVs contain DNA (EV-DNA) representing the entire genome and reflecting the mutational status of parental cells. However, the underlying mechanisms of DNA packaging into EVs and their functional consequence in human diseases including malignancy are still elusive. Methods: Here, via super-resolution imaging of single EVs and biochemical assay, we show that only 30 % of vesicles are DNA positive, and the majority of EV-DNA is presented on the surface of EVs. Histones associated with EVs are remarkably distinct from their cellular counterparts in their variants, size and post-translational modifications, indicating/suggesting unique chromatinization of EV-DNA. To identify factors essential for DNA loading on EVs, we designed a novel strategy of genome-wide CRISPR knockout screening in multiple cancer types. And performed in-vivo experiments to study how EV-DNA affects cancer progression.","journal":"Journal of Extracellular Vesicles","year":2023,"id":355626,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1104454,"name":"Kekkonen, Jaana","orcid":null,"position":1,"is_corresponding":false},{"id":1104455,"name":"Baek, Rikke","orcid":null,"position":2,"is_corresponding":false},{"id":1104456,"name":"Jørgensen, Malene Møller; id_orcid 0000-0003-1381-3863","orcid":null,"position":3,"is_corresponding":false},{"id":1104457,"name":"Siljander, Pia RM","orcid":null,"position":4,"is_corresponding":false},{"id":1104453,"name":"Puhka, Maija","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T01:13:21.145955Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}