{"doi":"10.1002/jev2.12062","title":"Cross‐flow microfiltration for isolation, selective capture and release of liposarcoma extracellular vesicles","abstract":"We present a resource-efficient approach to fabricate and operate a micro-nanofluidic device that uses cross-flow filtration to isolate and capture liposarcoma derived extracellular vesicles (EVs). The isolated extracellular vesicles were captured using EV-specific protein markers to obtain vesicle enriched media, which was then eluted for further analysis. Therefore, the micro-nanofluidic device integrates the unit operations of size-based separation with CD63 antibody immunoaffinity-based capture of extracellular vesicles in the same device to evaluate EV-cargo content for liposarcoma. The eluted media collected showed ∼76% extracellular vesicle recovery from the liposarcoma cell conditioned media and ∼32% extracellular vesicle recovery from dedifferentiated liposarcoma patient serum when compared against state-of-art extracellular vesicle isolation and subsequent quantification by ultracentrifugation. The results reported here also show a five-fold increase in amount of critical liposarcoma-relevant extracellular vesicle cargo obtained in 30 min presenting a significant advance over existing state-of-art.","journal":"Journal of Extracellular Vesicles","year":2021,"id":162039,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":678200,"name":"Adarsh Choudhury","orcid":"0000-0002-3288-9356","position":1,"is_corresponding":false},{"id":510822,"name":"Patricia Sarchet","orcid":null,"position":2,"is_corresponding":false},{"id":442426,"name":"Prashanth Mohana Sundaram","orcid":"0000-0002-9747-3060","position":3,"is_corresponding":false},{"id":483486,"name":"Gonzalo Lopez","orcid":"0000-0002-1337-5050","position":4,"is_corresponding":false},{"id":483483,"name":"Danielle Braggio","orcid":"0000-0002-3476-8179","position":5,"is_corresponding":false},{"id":679139,"name":"Gita Balakirsky","orcid":null,"position":6,"is_corresponding":false},{"id":483489,"name":"Raphael E. Pollock","orcid":"0000-0002-0443-1583","position":7,"is_corresponding":false},{"id":376759,"name":"Shaurya Prakash","orcid":"0000-0002-5263-0843","position":8,"is_corresponding":false},{"id":483488,"name":"Lucia Casadei","orcid":"0000-0002-5731-8197","position":0,"is_corresponding":true}],"reference_count":76,"raw_metadata":null,"created_at":"2026-07-18T23:45:00.600065Z","pmid":"33643547","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}