{"doi":"10.1002/jemt.20278","title":"Photoswitchable cyan fluorescent protein as a FRET donor","abstract":"<jats:title>Abstract</jats:title><jats:p>Among a variety of fluorescent proteins available today, there is a lack of suitable markers with excitation/emission in the violet/blue part of visible spectrum. Recently, we reported on photoswitchable cyan fluorescent protein (PS‐CFP), which represents monomeric high‐contrasting photactivatable label for in vivo protein movement tracking. However, PS‐CFP demands high intensity of light for the photoswitching. Therefore it can be employed as a common fluorescent tag at conventional light intensities, which cause negligible or zero photoactivation. High pH stability and unique positioning of excitation/emission peaks make it a worthy supplement to the existing palette of fluorescent proteins. Here we use PS‐CFP fusion with a yellow fluorescent protein phiYFP to show that PS‐CFP is a promising donor partner for the fluorescence resonance energy transfer (FRET). A remarkable phenomenon is that PS‐CFP donor fluorescence turned to be essentially stable with and without FRET, while acceptor emission demonstrated record dynamic range of up to 7.8‐fold. This makes the FRET pair presented a useful tool for the single color high throughput screenings. Here we also propose ways for further PS‐CFP enhancing, aiming to develop bright cyan fluorescent protein with unique spectral characteristics. Microsc. Res. Tech. 69:207–209, 2006. © 2006 Wiley‐Liss, Inc.</jats:p>","journal":"Microscopy Research and Technique","year":2006,"id":25433,"datarank":0.9530169671937057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.5682745635744751,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.5682745635744751,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":115276,"name":"Dmitriy M. Chudakov","orcid":null,"position":1,"is_corresponding":false},{"id":149653,"name":"Ekaterina A. Souslova","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16538627","pmcid":null,"openalex_id":"https://openalex.org/W2093169836","authors":[],"funders":[],"total_grants":0,"fwci":1.8694,"citation_percentile":0.87863002,"influential_citations":1,"citation_trend":[{"year":2012,"count":1},{"year":2021,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jemt.20278","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jemt.20278","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jemt.20278","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.20278","host_type":"publisher"},{"url":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.20278","host_type":"publisher"},{"url":"https://doi.org/10.1002/jemt.20278","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16538627","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Photochromic and Fluorescence Chemistry","Photoreceptor and optogenetics research","Medicine","Chemistry","Biology","Materials Science","Bacterial Proteins","Escherichia coli Proteins","Fluorescence Resonance Energy Transfer","Green Fluorescent Proteins","Light","Luminescent Proteins","Recombinant Fusion Proteins"],"mesh_terms":["Bacterial Proteins","Light","Luminescent Proteins","Recombinant Fusion Proteins","Escherichia coli Proteins","Fluorescence Resonance Energy Transfer","Green Fluorescent Proteins"],"keywords":["Cyan","Förster resonance energy transfer","Fluorescence","Yellow fluorescent protein","Fluorescent protein","Chemistry","Photochemistry","Green fluorescent protein","Biophysics","Optics","Biology","Biochemistry","Physics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T04:35:54.354782Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}