{"doi":"10.1002/jcp.31133","title":"Effect of stretching on inflammation in a subcutaneous carrageenan mouse model analyzed at single‐cell resolution","abstract":"Abstract Understanding the factors that influence the biological response to inflammation is crucial, due to its involvement in physiological and pathological processes, including tissue repair/healing, cancer, infections, and autoimmune diseases. We have previously demonstrated that in vivo stretching can reduce inflammation and increase local pro‐resolving lipid mediators in rats, suggesting a direct mechanical effect on inflammation resolution. Here we aimed to explore further the effects of stretching at the cellular/molecular level in a mouse subcutaneous carrageenan‐inflammation model. Stretching for 10 min twice a day reduced inflammation, increased the production of pro‐resolving mediator pathway intermediate 17‐HDHA at 48 h postcarrageenan injection, and decreased both pro‐resolving and pro‐inflammatory mediators (e.g., PGE 2 and PGD 2 ) at 96 h. Single‐cell RNA sequencing analysis of inflammatory lesions at 96 h showed that stretching increased the expression of both pro‐inflammatory ( Nos2 ) and pro‐resolution ( Arg1 ) genes in M1 and M2 macrophages at 96 h. An intercellular communication analysis predicted specific ligand–receptor interactions orchestrated by neutrophils and M2a macrophages, suggesting a continuous neutrophil presence recruiting immune cells such as activated macrophages to contain the antigen while promoting resolution and preserving tissue homeostasis.","journal":"Journal of Cellular Physiology","year":2023,"id":350329,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":920429,"name":"Dennis Muñoz‐Vergara","orcid":"0000-0002-4680-5408","position":1,"is_corresponding":false},{"id":302989,"name":"Daniel Martı́n","orcid":"0000-0002-8880-9087","position":2,"is_corresponding":false},{"id":1016224,"name":"Rebecca Thompson","orcid":"0009-0006-6734-2138","position":3,"is_corresponding":false},{"id":677049,"name":"Brian E. Sansbury","orcid":"0000-0002-6339-2940","position":4,"is_corresponding":false},{"id":677052,"name":"Matthew Spite","orcid":"0000-0002-8868-3648","position":5,"is_corresponding":false},{"id":417223,"name":"Gary J. Badger","orcid":null,"position":6,"is_corresponding":false},{"id":218669,"name":"Hélène M. Langevin","orcid":"0000-0002-3142-1671","position":7,"is_corresponding":false},{"id":1094249,"name":"Lisbeth Berrueta","orcid":"0000-0002-5674-6448","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T01:12:23.769679Z","pmid":"37909412","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}