{"doi":"10.1002/jcp.31061","title":"Identification of common sequence motifs shared exclusively among selectively packed exosomal pathogenic microRNAs during rickettsial infections","abstract":"We previously reported that microRNA (miR)23a and miR30b are selectively sorted into exosomes derived from rickettsia-infected endothelial cells (R-ECExos). Yet, the mechanism remains unknown. Cases of spotted fever rickettsioses have been increasing, and infections with these bacteria cause life-threatening diseases by targeting brain and lung tissues. Therefore, the goal of the present study is to further dissect the molecular mechanism underlying R-ECExos-induced barrier dysfunction of normal recipient microvascular endothelial cells (MECs), depending on their exosomal RNA cargos. Infected ticks transmit the rickettsiae to human hosts following a bite and injections of the bacteria into the skin. In the present study, we demonstrate that treatment with R-ECExos, which were derived from spotted fever group R parkeri infected human dermal MECs, induced disruptions of the paracellular adherens junctional protein VE-cadherin, and breached the paracellular barrier function in recipient pulmonary MECs (PMECs) in an exosomal RNA-dependent manner. We did not detect different levels of miRs in parent dermal MECs following rickettsial infections. However, we demonstrated that the microvasculopathy-relevant miR23a-27a-24 cluster and miR30b are selectively enriched in R-ECExos. Bioinformatic analysis revealed that common sequence motifs are shared exclusively among the exosomal, selectively-enriched miR23a cluster and miR30b at different levels. Taken together, these data warrant further functional identification and characterization of a monopartition, bipartition, or tripartition among ACA, UCA, and CAG motifs that guide recognition of microvasculopathy-relevant miR23a-27a-24 and miR30b, and subsequently results in their selective enrichments in R-ECExos.","journal":"Journal of Cellular Physiology","year":2023,"id":384388,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":568607,"name":"Yuan Qiu","orcid":"0000-0002-8997-4367","position":1,"is_corresponding":false},{"id":691692,"name":"Diane C. Cockrell","orcid":null,"position":2,"is_corresponding":false},{"id":453509,"name":"Qing Chang","orcid":"0000-0003-4122-3705","position":3,"is_corresponding":false},{"id":1151953,"name":"Sorosh Husseinzadeh","orcid":null,"position":4,"is_corresponding":false},{"id":480474,"name":"Changcheng Zhou","orcid":"0000-0001-7649-0710","position":5,"is_corresponding":false},{"id":503238,"name":"Xiang Fang","orcid":"0000-0003-4094-894X","position":6,"is_corresponding":false},{"id":326649,"name":"Xiaoyong Bao","orcid":"0000-0002-6884-2584","position":7,"is_corresponding":false},{"id":284814,"name":"Yang Jin","orcid":"0000-0002-4779-531X","position":8,"is_corresponding":false},{"id":563206,"name":"Angelo Gaitas","orcid":"0000-0003-4428-3949","position":9,"is_corresponding":false},{"id":275505,"name":"Kamil Khanipov","orcid":"0000-0002-3881-737X","position":10,"is_corresponding":false},{"id":453514,"name":"Taís B. Saito","orcid":"0000-0002-0337-4441","position":11,"is_corresponding":false},{"id":453515,"name":"Bin Gong","orcid":"0000-0002-3929-3897","position":12,"is_corresponding":false},{"id":454228,"name":"Jiani Bei","orcid":null,"position":0,"is_corresponding":true}],"reference_count":111,"raw_metadata":null,"created_at":"2026-07-19T01:17:33.041385Z","pmid":"37334929","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}