{"doi":"10.1002/jcp.30942","title":"Novel differential calcium regulation of hematopoietic stem and progenitor cells under physiological low oxygen conditions","abstract":"Abstract Low oxygen bone marrow (BM) niches (~1%–4% low O 2 ) provide critical signals for hematopoietic stem/progenitor cells (HSC/HSPCs). Our presented data are the first to investigate live, sorted HSC/HSPCs in their native low O 2 conditions. Transcriptional and proteomic analysis uncovered differential Ca 2+ regulation that correlated with overlapping phenotypic populations consisting of robust increases of cytosolic and mitochondrial Ca 2+ , ABC transporter (ABCG2) expression and sodium/hydrogen exchanger (NHE1) expression in live, HSC/HSPCs remaining in constant low O 2. We identified a novel Ca 2+ high population in HSPCs predominantly detected in low O 2 that displayed enhanced frequency of phenotypic LSK/LSKCD150 in low O 2 replating assays compared to Ca 2+ low populations. Inhibition of the Ca 2+ regulator NHE1 (Cariporide) resulted in attenuation of both the low O 2 induced Ca 2+ high population and subsequent enhanced maintenance of phenotypic LSK and LSKCD150 during low O 2 replating. These data reveal multiple levels of differential Ca 2+ regulation in low O 2 resulting in phenotypic, signaling, and functional consequences in HSC/HSPCs.","journal":"Journal of Cellular Physiology","year":2023,"id":388111,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9463,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1157364,"name":"Melissa Hartman","orcid":"0000-0001-6923-804X","position":1,"is_corresponding":false},{"id":1157729,"name":"Jacob Slack","orcid":null,"position":2,"is_corresponding":false},{"id":1157730,"name":"Christopher Basile","orcid":null,"position":3,"is_corresponding":false},{"id":294284,"name":"Sheng Liu","orcid":"0000-0002-2497-8666","position":4,"is_corresponding":false},{"id":300846,"name":"Jun Wan","orcid":"0000-0001-9286-6562","position":5,"is_corresponding":false},{"id":1006868,"name":"Heather A. O’Leary","orcid":"0000-0003-1321-1520","position":6,"is_corresponding":false},{"id":1157728,"name":"Paige Dausinas Ni","orcid":null,"position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T01:18:13.977537Z","pmid":"37051890","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}