{"doi":"10.1002/jcb.24994","title":"PPAR‐γ Promotes Endothelial Cell Migration By Inducing the Expression of Sema3g","abstract":"<jats:title>ABSTRACT</jats:title><jats:sec><jats:label/><jats:p>In addition to regulating lipid and glucose metabolism, the nuclear receptor PPAR‐γ has emerged as a potentially relevant player in regulating endothelial cell function. Despite the identification of numerous PPAR‐γ targets involved in vascular development, the targets downstream of PPAR‐γ that directly affect endothelial cell function remain to be elucidated. In this report, we identify Sema3g as a novel PPAR‐γ‐regulated gene playing a substantial role in endothelial biology, particularly with respect to endothelial cell migration. Sema3g expression is induced by either overexpression of PPAR‐γ or PPAR‐γ ligands treatment in human umbilical vein endothelial cells (HUVECs). Chromatin immunoprecipitation (ChIP) and transient transfection assays revealed that PPAR‐γ binds to the Sema3g promoter and activates transcription. Furthermore, we show that overexpression of Sema3g augments PPAR‐γ‐driven HUVECs migration, whereas silencing of Sema3g expression almost completely abrogates PPAR‐γ or Sema3g‐mediated cell migration. Accordingly, the anti‐neuropilin‐2 (Sema3g receptor) neutralizing antibody treatment markedly inhibits Sema3g‐induced cell migration. Collectively, these results identify Sema3g as one of the downstream effectors of PPAR‐γ, which is centrally involved in regulating endothelial cell migration. J. Cell. Biochem. 116: 514–523, 2015. © 2014 Wiley Periodicals, Inc.</jats:p></jats:sec>","journal":"Journal of Cellular Biochemistry","year":2015,"id":646628,"datarank":0.5244761342199721,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.0,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":540839,"name":"Jingjin Li","orcid":"0000-0002-2129-5122","position":1,"is_corresponding":false},{"id":514938,"name":"Min Liu","orcid":"0000-0001-8115-5407","position":2,"is_corresponding":false},{"id":527496,"name":"Hong Zhang","orcid":"0000-0002-6417-8399","position":3,"is_corresponding":false},{"id":1084570,"name":"Nanping Wang","orcid":"0000-0002-8528-8132","position":4,"is_corresponding":false},{"id":199213,"name":"Weiwei Liu","orcid":"0000-0001-5082-9114","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"PPAR‐γ Promotes Endothelial Cell Migration By Inducing the Expression of Sema3g","abstract":"<jats:title>ABSTRACT</jats:title><jats:sec><jats:label/><jats:p>In addition to regulating lipid and glucose metabolism, the nuclear receptor PPAR‐γ has emerged as a potentially relevant player in regulating endothelial cell function. Despite the identification of numerous PPAR‐γ targets involved in vascular development, the targets downstream of PPAR‐γ that directly affect endothelial cell function remain to be elucidated. In this report, we identify Sema3g as a novel PPAR‐γ‐regulated gene playing a substantial role in endothelial biology, particularly with respect to endothelial cell migration. Sema3g expression is induced by either overexpression of PPAR‐γ or PPAR‐γ ligands treatment in human umbilical vein endothelial cells (HUVECs). Chromatin immunoprecipitation (ChIP) and transient transfection assays revealed that PPAR‐γ binds to the Sema3g promoter and activates transcription. Furthermore, we show that overexpression of Sema3g augments PPAR‐γ‐driven HUVECs migration, whereas silencing of Sema3g expression almost completely abrogates PPAR‐γ or Sema3g‐mediated cell migration. Accordingly, the anti‐neuropilin‐2 (Sema3g receptor) neutralizing antibody treatment markedly inhibits Sema3g‐induced cell migration. Collectively, these results identify Sema3g as one of the downstream effectors of PPAR‐γ, which is centrally involved in regulating endothelial cell migration. J. Cell. Biochem. 116: 514–523, 2015. © 2014 Wiley Periodicals, Inc.</jats:p></jats:sec>","is_dataset_classified":null,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25335934","pmcid":null,"openalex_id":"https://openalex.org/W1893984031","authors":[],"funders":[{"funder_name":"National Science Foundation of China","grant_id":"30600231","title":null},{"funder_name":"National Science Foundation of China","grant_id":"81270951","title":null}],"total_grants":2,"fwci":0.26,"citation_percentile":0.54743779,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":3},{"year":2021,"count":12},{"year":2022,"count":4},{"year":2023,"count":4},{"year":2024,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjcb.24994","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.24994","host_type":"publisher"},{"url":"https://doi.org/10.1002/jcb.24994","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25335934","host_type":"repository"}],"fields_of_study":["Angiogenesis and VEGF in Cancer","Hippo pathway signaling and YAP/TAZ","Protein Degradation and Inhibitors","Cell Movement","Endothelial Cells","Human Umbilical Vein Endothelial Cells","Humans","PPAR gamma","Promoter Regions, Genetic","Rosiglitazone","Semaphorins","Thiazolidinediones","Up-Regulation"],"mesh_terms":["Rosiglitazone","Cell Movement","Humans","Promoter Regions, Genetic","Up-Regulation","Semaphorins","Endothelial Cells","Thiazolidinediones","PPAR gamma","Human Umbilical Vein Endothelial Cells"],"keywords":["Chromatin immunoprecipitation","Cell biology","Cell migration","Endothelial stem cell","Gene silencing","Peroxisome proliferator-activated receptor","Umbilical vein","Transfection","Biology","Transcription factor","Nuclear receptor","Human umbilical vein endothelial cell","Cell","Receptor","Chemistry","Cell culture","Gene expression","Promoter","Biochemistry","Gene","In vitro","Migration","Transactivation","Endothelial Cell","Ppar-γ","Semaphorin3g"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T14:07:17.396448Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}