{"doi":"10.1002/jcb.22475","title":"The 19S proteasomal lid subunit POH1 enhances the transcriptional activation by Mitf in osteoclasts","abstract":"<jats:title>Abstract</jats:title><jats:p>The microphthalmia‐associated transcription factor (Mitf) regulates gene expression required for osteoclast differentiation. Genes regulated by Mitf have been previously identified. However, proteins that interact and regulate Mitf's activity in osteoclasts are not well known. Here, we report that POH1, a subunit of the 19S proteasome lid is a regulator of Mitf. We show that POH1 and Mitf interact in osteoclasts and that this interaction is dependent on RANKL signaling. Overexpression of POH1 increased Mitf's activation of 5XGal4‐TK and <jats:italic>Acp5</jats:italic> promoters. The amino terminus of POH1 mediates the binding to Mitf and is sufficient to increase Mitf's transcriptional activity. Finally, we show that mutations in the JAMM motif of POH1 reduced Mitf activation of promoters. In summary, our results identify a novel mechanism of Mitf regulation in osteoclasts by POH1. J. Cell. Biochem. 109: 967–974, 2010. © 2010 Wiley‐Liss, Inc.</jats:p>","journal":"Journal of Cellular Biochemistry","year":2010,"id":629997,"datarank":0.5101796072493234,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"self_citation_contribution":0.5101796072493234,"citation_network_contribution":0.0,"self_endowment_contribution":0.5101796072493234,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1631902,"name":"Chee Sohn","orcid":null,"position":1,"is_corresponding":false},{"id":1631903,"name":"Bria Kaiser","orcid":null,"position":2,"is_corresponding":false},{"id":372452,"name":"Eric D. Jensen","orcid":"0000-0003-0323-9706","position":3,"is_corresponding":false},{"id":361558,"name":"Kim C. Mansky","orcid":"0000-0002-2826-5030","position":4,"is_corresponding":false},{"id":1631901,"name":"Toni Schwarz","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The 19S proteasomal lid subunit POH1 enhances the transcriptional activation by Mitf in osteoclasts","abstract":"<jats:title>Abstract</jats:title><jats:p>The microphthalmia‐associated transcription factor (Mitf) regulates gene expression required for osteoclast differentiation. Genes regulated by Mitf have been previously identified. However, proteins that interact and regulate Mitf's activity in osteoclasts are not well known. Here, we report that POH1, a subunit of the 19S proteasome lid is a regulator of Mitf. We show that POH1 and Mitf interact in osteoclasts and that this interaction is dependent on RANKL signaling. Overexpression of POH1 increased Mitf's activation of 5XGal4‐TK and <jats:italic>Acp5</jats:italic> promoters. The amino terminus of POH1 mediates the binding to Mitf and is sufficient to increase Mitf's transcriptional activity. Finally, we show that mutations in the JAMM motif of POH1 reduced Mitf activation of promoters. In summary, our results identify a novel mechanism of Mitf regulation in osteoclasts by POH1. J. Cell. Biochem. 109: 967–974, 2010. © 2010 Wiley‐Liss, Inc.</jats:p>","is_dataset_classified":null,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20058232","pmcid":null,"openalex_id":"https://openalex.org/W1991426200","authors":[],"funders":[],"total_grants":0,"fwci":0.6712,"citation_percentile":0.67922581,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":4},{"year":2019,"count":5},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjcb.22475","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.22475","host_type":"publisher"},{"url":"https://doi.org/10.1002/jcb.22475","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20058232","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3273965","host_type":"repository"}],"fields_of_study":["Ubiquitin and proteasome pathways","NF-κB Signaling Pathways","Retinoids in leukemia and cellular processes"],"mesh_terms":["Amino Acid Sequence","Animals","Cell Differentiation","Humans","Macrophages","Molecular Sequence Data","Osteoclasts","Protein Binding","Protein Processing, Post-Translational","Transcriptional Activation","Trans-Activators","Protein Structure, Tertiary","Jurkat Cells","Two-Hybrid System Techniques","Amino Acid Motifs","Protein Subunits","NIH 3T3 Cells","Proteasome Endopeptidase Complex","Mice","Microphthalmia-Associated Transcription Factor","Ubiquitination"],"keywords":["Microphthalmia-associated transcription factor","IRF4","Transcription factor","Protein subunit","Cell biology","RANKL","Biology","Gene","Chemistry","Genetics","Activator (genetics)"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T20:14:27.128675Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}