{"doi":"10.1002/jbmr.3975","title":"β-Catenin Preserves the Stem State of Murine Bone Marrow Stromal Cells Through Activation of EZH2","abstract":"During bone marrow stromal cell (BMSC) differentiation, both Wnt signaling and the development of a rigid cytoskeleton promote commitment to the osteoblastic over adipogenic lineage. β-catenin plays a critical role in the Wnt signaling pathway to facilitate downstream effects on gene expression. We show that β-catenin was additive with cytoskeletal signals to prevent adipogenesis, and β-catenin knockdown promoted adipogenesis even when the actin cytoskeleton was depolymerized. β-catenin also prevented osteoblast commitment in a cytoskeletal-independent manner, with β-catenin knockdown enhancing lineage commitment. Chromatin immunoprecipitation (ChIP)-sequencing demonstrated binding of β-catenin to the promoter of enhancer of zeste homolog 2 (EZH2), a key component of the polycomb repressive complex 2 (PRC2) complex that catalyzes histone methylation. Knockdown of β-catenin reduced EZH2 protein levels and decreased methylated histone 3 (H3K27me3) at osteogenic loci. Further, when EZH2 was inhibited, β-catenin's anti-differentiation effects were lost. These results indicate that regulating EZH2 activity is key to β-catenin's effects on BMSCs to preserve multipotentiality. © 2020 American Society for Bone and Mineral Research.","journal":"Journal of Bone and Mineral Research","year":2020,"id":65723,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":275541,"name":"Christopher R. Paradise","orcid":"0000-0002-5882-709X","position":1,"is_corresponding":false},{"id":348009,"name":"Zhihui Xie","orcid":"0000-0002-1556-0700","position":2,"is_corresponding":false},{"id":348010,"name":"Jeyantt Srinivas Sankaran","orcid":"0000-0002-8613-9219","position":3,"is_corresponding":false},{"id":348011,"name":"Gunes Uzer","orcid":"0000-0002-1178-4942","position":4,"is_corresponding":false},{"id":348012,"name":"Maya Styner","orcid":"0000-0001-9790-8645","position":5,"is_corresponding":false},{"id":348013,"name":"Mark B. Meyer","orcid":"0000-0001-6612-7491","position":6,"is_corresponding":false},{"id":275539,"name":"Amel Dudakovic","orcid":"0000-0002-8850-3977","position":7,"is_corresponding":false},{"id":275536,"name":"André J. van Wijnen","orcid":"0000-0002-4458-0946","position":8,"is_corresponding":false},{"id":348014,"name":"Janet Rubin","orcid":"0000-0003-3534-8667","position":9,"is_corresponding":false},{"id":348008,"name":"Buer Sen","orcid":"0000-0002-0487-7817","position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":null,"created_at":"2026-07-18T21:13:43.728351Z","pmid":"32022326","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}