{"doi":"10.1002/jbm.a.37922","title":"A Rapid Manual Solid Phase Peptide Synthesis Method for High‐Throughput Peptide Production","abstract":"Solid phase peptide synthesis (SPPS) techniques are critical for developing and using peptides in various biomedical applications. However, typical synthesis routes used in SPPS are either resource-intensive (e.g., with automated synthesis or commercial services) or time-consuming (e.g., with manual benchtop synthesis). Here, a rapid manual synthesis method was developed to produce up to 8 peptides with fast cycle times simultaneously. Peptides synthesized manually were of equivalent or superior quality to those produced by in-house microwave-assisted automated peptide synthesis, with higher average crude purity of 70% compared to 50%. The method significantly reduced synthesis time, enabling the parallel coupling of up to 8 amino acids simultaneously in 15-20 min, as opposed to traditional benchtop peptide synthesis, which requires 80-150 min per amino acid. This approach offers an intermediate throughput between milligram-scale libraries and gram-scale single peptide synthesis, enabling rapid iteration for novel peptide designs without the need for expensive automated systems. As a result, peptide modifications, including incorporation of unnatural amino acids, can be explored, accelerating the development of peptides for a wide range of applications.","journal":"Journal of Biomedical Materials Research Part A","year":2025,"id":525655,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":921930,"name":"Brittany L. Abraham","orcid":"0000-0001-9147-8541","position":1,"is_corresponding":false},{"id":1400795,"name":"Emmanuella Adjei‐Sowah","orcid":null,"position":2,"is_corresponding":false},{"id":1400796,"name":"Alyson March","orcid":null,"position":3,"is_corresponding":false},{"id":1400364,"name":"Kevin Ling","orcid":"0000-0002-4872-5751","position":4,"is_corresponding":false},{"id":705914,"name":"Sayantani Basu","orcid":null,"position":5,"is_corresponding":false},{"id":303880,"name":"Danielle S. W. Benoit","orcid":"0000-0001-7137-8164","position":6,"is_corresponding":false},{"id":415364,"name":"Clyde Overby","orcid":"0000-0001-7033-280X","position":0,"is_corresponding":true}],"reference_count":23,"raw_metadata":null,"created_at":"2026-07-19T02:50:21.274116Z","pmid":"40312963","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}