{"doi":"10.1002/jbio.200710024","title":"Fluorescent proteins for single‐molecule fluorescence applications","abstract":"<jats:title>Abstract</jats:title><jats:p>We present single‐molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single‐molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single‐molecule applications, demonstrated by high photostability and rare fluorescence‐intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single‐molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</jats:p>","journal":"Journal of Biophotonics","year":2008,"id":47306,"datarank":2.433736781964191,"base_score":4.304065093204169,"endowment":4.304065093204169,"self_citation_contribution":0.6456097639806255,"citation_network_contribution":1.788127017983565,"self_endowment_contribution":0.6456097639806255,"citer_contribution":1.788127017983565,"corpus_percentile":null,"corpus_rank":null,"citation_count":73,"citer_count":64,"citers_with_citation_signal":51,"citers_with_endowment":51,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":218720,"name":"Robert Kasper","orcid":null,"position":1,"is_corresponding":false},{"id":218721,"name":"Thorsten Seidel","orcid":null,"position":2,"is_corresponding":false},{"id":218722,"name":"Philip Tinnefeld","orcid":null,"position":3,"is_corresponding":false},{"id":218723,"name":"Karl‐Josef Dietz","orcid":null,"position":4,"is_corresponding":false},{"id":218724,"name":"Mike Heilemann","orcid":null,"position":5,"is_corresponding":false},{"id":44852,"name":"Markus Sauer","orcid":"0000-0002-1692-3219","position":6,"is_corresponding":false},{"id":218719,"name":"Britta Seefeldt","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Fluorescent proteins for single‐molecule fluorescence applications","abstract":"<jats:title>Abstract</jats:title><jats:p>We present single‐molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single‐molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single‐molecule applications, demonstrated by high photostability and rare fluorescence‐intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single‐molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</jats:p>","is_dataset_classified":null,"base_score":4.304065093204169,"endowment":4.304065093204169,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19343637","pmcid":null,"openalex_id":"https://openalex.org/W2054530395","authors":[],"funders":[],"total_grants":0,"fwci":6.5703,"citation_percentile":0.9624871,"influential_citations":1,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":7},{"year":2014,"count":3},{"year":2015,"count":4},{"year":2016,"count":3},{"year":2017,"count":3},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":4},{"year":2021,"count":6},{"year":2022,"count":4},{"year":2023,"count":6},{"year":2025,"count":9},{"year":2026,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbio.200710024","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbio.200710024","host_type":"publisher"},{"url":"https://doi.org/10.1002/jbio.200710024","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19343637","host_type":"repository"},{"url":"https://pub.uni-bielefeld.de/record/1636544","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Advanced Electron Microscopy Techniques and Applications","Advanced Biosensing Techniques and Applications","Chemistry","Medicine","Biology","Materials Science","Fluorescence Resonance Energy Transfer","Green Fluorescent Proteins","Microscopy, Fluorescence","Recombinant Fusion Proteins","Spectrometry, Fluorescence"],"mesh_terms":["Microscopy, Fluorescence","Recombinant Fusion Proteins","Spectrometry, Fluorescence","Fluorescence Resonance Energy Transfer","Green Fluorescent Proteins"],"keywords":["mCherry","Fluorescence","Fluorescence in the life sciences","Förster resonance energy transfer","Fluorophore","Green fluorescent protein","Biophysics","Chemistry","Bimolecular fluorescence complementation","Fluorescence microscope","Single-molecule experiment","Fluorescence-lifetime imaging microscopy","Molecule","Biomolecule","Quantum yield","Biology","Biochemistry","Physics","Optics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T14:53:06.620476Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}