{"doi":"10.1002/ijc.34241","title":"<scp>One‐stop</scp> molecular classification of endometrial carcinoma using comprehensive <scp>next‐generation</scp> sequencing","abstract":"<jats:title>Abstract</jats:title><jats:p>This study aims to investigate the feasibility of molecular classification using only comprehensive next‐generation sequencing‐based techniques and its relationship with survival outcomes in patients with endometrial cancer. Paired tumor‐normal sequencing data of 1021 cancer‐related genes using tumor tissues or peripheral blood samples and clinical data were retrospectively collected from a cohort of endometrial cancers. The microsatellite instability status was inferred using the MSIsensor (v0.5) with a cut‐off of 8%. Sixty patients were classified into four groups: POLE<jats:sup>MUT</jats:sup> group (13.3%), MSI‐H group (20%), TP53<jats:sup>WT</jats:sup> group (45%) and TP53<jats:sup>MUT</jats:sup> group (21.7%). Patients within TP53<jats:sup>MUT</jats:sup> group were more common in serous carcinoma compared to endometrioid carcinoma (<jats:italic>P</jats:italic> = .0098). TP53WT was significantly correlated with early stage and low grade. TP53<jats:sup>MUT</jats:sup> group was associated with significantly worse DFS compared to MSI‐H group and TP53<jats:sup>WT</jats:sup> group (<jats:italic>P</jats:italic> = .014 and .004, respectively). Comprehensive next‐generation sequencing is a reliable and simple method to stratify the prognosis of endometrial carcinoma. It can be potentially used to guide treatment of patients with endometrial cancer in routine practice.</jats:p>","journal":"International Journal of Cancer","year":2022,"id":588980,"datarank":0.6667034003097463,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.29396740284154615,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.29396740284154615,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":8,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1387589,"name":"Rui Chen","orcid":"0000-0002-0445-7847","position":1,"is_corresponding":false},{"id":1506897,"name":"Mingming Yuan","orcid":null,"position":2,"is_corresponding":false},{"id":761966,"name":"Dong Wang","orcid":"0000-0002-8139-8502","position":3,"is_corresponding":false},{"id":1506898,"name":"Chunling Fu","orcid":null,"position":4,"is_corresponding":false},{"id":35348,"name":"Rongrong Chen","orcid":"0000-0002-4489-4179","position":5,"is_corresponding":false},{"id":1506899,"name":"Cuirong Lei","orcid":null,"position":6,"is_corresponding":false},{"id":1189068,"name":"Qi Zhou","orcid":"0000-0001-5673-4263","position":7,"is_corresponding":false},{"id":1506896,"name":"Yucong Li","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<scp>One‐stop</scp> molecular classification of endometrial carcinoma using comprehensive <scp>next‐generation</scp> sequencing","abstract":"<jats:title>Abstract</jats:title><jats:p>This study aims to investigate the feasibility of molecular classification using only comprehensive next‐generation sequencing‐based techniques and its relationship with survival outcomes in patients with endometrial cancer. Paired tumor‐normal sequencing data of 1021 cancer‐related genes using tumor tissues or peripheral blood samples and clinical data were retrospectively collected from a cohort of endometrial cancers. The microsatellite instability status was inferred using the MSIsensor (v0.5) with a cut‐off of 8%. Sixty patients were classified into four groups: POLE<jats:sup>MUT</jats:sup> group (13.3%), MSI‐H group (20%), TP53<jats:sup>WT</jats:sup> group (45%) and TP53<jats:sup>MUT</jats:sup> group (21.7%). Patients within TP53<jats:sup>MUT</jats:sup> group were more common in serous carcinoma compared to endometrioid carcinoma (<jats:italic>P</jats:italic> = .0098). TP53WT was significantly correlated with early stage and low grade. TP53<jats:sup>MUT</jats:sup> group was associated with significantly worse DFS compared to MSI‐H group and TP53<jats:sup>WT</jats:sup> group (<jats:italic>P</jats:italic> = .014 and .004, respectively). Comprehensive next‐generation sequencing is a reliable and simple method to stratify the prognosis of endometrial carcinoma. It can be potentially used to guide treatment of patients with endometrial cancer in routine practice.</jats:p>","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36036365","pmcid":null,"openalex_id":"https://openalex.org/W4291222669","authors":[],"funders":[],"total_grants":0,"fwci":1.7701,"citation_percentile":0.84730287,"influential_citations":0,"citation_trend":[{"year":2023,"count":6},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijc.34241","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijc.34241","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.34241","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ijc.34241","host_type":"publisher"},{"url":"https://doi.org/10.1002/ijc.34241","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36036365","host_type":"repository"}],"fields_of_study":["Endometrial and Cervical Cancer Treatments","Ovarian cancer diagnosis and treatment","Sarcoma Diagnosis and Treatment","Biomarkers, Tumor","Carcinoma, Endometrioid","Endometrial Neoplasms","Female","High-Throughput Nucleotide Sequencing","Humans","Microsatellite Instability","Mutation","Prognosis","Retrospective Studies"],"mesh_terms":["Female","Humans","Mutation","Prognosis","Retrospective Studies","Biomarkers, Tumor","Endometrial Neoplasms","Carcinoma, Endometrioid","Microsatellite Instability","High-Throughput Nucleotide Sequencing"],"keywords":["Endometrial cancer","Microsatellite instability","Carcinoma","Serous fluid","Medicine","Serous carcinoma","Oncology","Internal medicine","Stage (stratigraphy)","Cancer","Cohort","Gynecology","Gene","Microsatellite","Biology","Ovarian cancer","Genetics","Endometrial carcinoma","Molecular classification","Next-generation Sequencing","Disease-free Survival","Prognosis Stratification"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T11:07:11.205550Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}