{"doi":"10.1002/ijc.2910610423","title":"B‐cell lymphomagenesis in SIV‐immunosuppressed cynomolgus monkeys","abstract":"<jats:title>Abstract</jats:title><jats:p>B‐cell lymphomas developed frequently (approx. 40%) in SIVsm (SMM3) immunosuppressed monkeys and were mostly extranodal, aggressive and all associated with an EBV‐related simian herpes virus operationally designated herpes virus <jats:italic>Macoca fascicularis</jats:italic> (HVMF‐1). Lymphoma tissues from 21 monkeys were studied by PCR and DNA PAGE for mono/oligoclonality of the VDJ‐rearranged IgH genes. Most lymphomas (n = 15) showed a monoclonal and approximately 1/3 (n = 6) an oligoclonal VDJ rearrangement pattern. The time after infection to tumor presentation was significantly shorter for oligoclonal than for monoclonal lymphomas, suggesting that oligoclonal selection frequently precedes the outgrowth of a single malignant clone. Comparison of the VDJ rearrangements in an established lymphoma cell line and the original, oligoclonal lymphoma tissue indicated <jats:italic>in vitro</jats:italic> selection of one HVMF‐infected clone. Longitudinal studies of sequential lymph‐node biopsies showed that the malignant lymphoma clone in 3 out of 8 lymphomas could be identified as a predominant clone in lymph nodes 2‐12 months after SIV infection and 6‐10 months before clinical presentation of the lymphomas. VDJ‐rearranged DNA corresponding to that of the lymphomas was also detected in most sera at the time of lymphoma manifestation but not in corresponding PBL preparations. Clearly, the SIVsm AIDS model in cynomolgus monkeys represents a powerful tool for biological and clinical studies of herpes‐virus‐associated lymphomagenesis in immunosuppressed states. © <jats:italic>1995 Wiley‐Liss, Inc</jats:italic>.</jats:p>","journal":"International Journal of Cancer","year":1995,"id":622715,"datarank":0.5333022092234121,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":0.0,"self_endowment_contribution":0.5333022092234121,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1609112,"name":"Ephata E. 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Lymphoma tissues from 21 monkeys were studied by PCR and DNA PAGE for mono/oligoclonality of the VDJ‐rearranged IgH genes. Most lymphomas (n = 15) showed a monoclonal and approximately 1/3 (n = 6) an oligoclonal VDJ rearrangement pattern. The time after infection to tumor presentation was significantly shorter for oligoclonal than for monoclonal lymphomas, suggesting that oligoclonal selection frequently precedes the outgrowth of a single malignant clone. Comparison of the VDJ rearrangements in an established lymphoma cell line and the original, oligoclonal lymphoma tissue indicated <jats:italic>in vitro</jats:italic> selection of one HVMF‐infected clone. Longitudinal studies of sequential lymph‐node biopsies showed that the malignant lymphoma clone in 3 out of 8 lymphomas could be identified as a predominant clone in lymph nodes 2‐12 months after SIV infection and 6‐10 months before clinical presentation of the lymphomas. VDJ‐rearranged DNA corresponding to that of the lymphomas was also detected in most sera at the time of lymphoma manifestation but not in corresponding PBL preparations. Clearly, the SIVsm AIDS model in cynomolgus monkeys represents a powerful tool for biological and clinical studies of herpes‐virus‐associated lymphomagenesis in immunosuppressed states. © <jats:italic>1995 Wiley‐Liss, Inc</jats:italic>.</jats:p>","is_dataset_classified":null,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7759163","pmcid":null,"openalex_id":"https://openalex.org/W1975932113","authors":[],"funders":[],"total_grants":0,"fwci":0.9362,"citation_percentile":0.76845027,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2014,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fijc.2910610423","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.2910610423","host_type":"publisher"},{"url":"https://doi.org/10.1002/ijc.2910610423","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7759163","host_type":"repository"}],"fields_of_study":["Viral-associated cancers and disorders","Herpesvirus Infections and Treatments","Cytomegalovirus and herpesvirus research","Animals","Base Sequence","Biopsy","Chlorocebus aethiops","DNA, Neoplasm","Gene Rearrangement, B-Lymphocyte","Genes, Immunoglobulin","Herpesviridae","Herpesvirus 4, Human","Humans","Immunocompromised Host","In Situ Hybridization","Lymph Nodes","Lymphoma, B-Cell","Macaca fascicularis","Molecular Sequence Data","Polymerase Chain Reaction","RNA, Viral","RNA-Binding Proteins","Sensitivity and Specificity","Simian Acquired Immunodeficiency Syndrome","Simian Immunodeficiency Virus","Vero Cells"],"mesh_terms":["Animals","Base Sequence","Biopsy","Chlorocebus aethiops","DNA, Neoplasm","Herpesvirus 4, Human","Genes, Immunoglobulin","Herpesviridae","Humans","Lymph Nodes","Macaca fascicularis","Molecular Sequence Data","RNA, Viral","Sensitivity and Specificity","Vero Cells","Simian Immunodeficiency Virus","Gene Rearrangement, B-Lymphocyte","Simian Acquired Immunodeficiency Syndrome","Polymerase Chain Reaction","Lymphoma, B-Cell","RNA-Binding Proteins","Immunocompromised Host","In Situ Hybridization","Simian immunodeficiency virus"],"keywords":["clone (Java method)","Lymphoma","Virology","Biology","Virus","Monoclonal","Lymph node","Monoclonal antibody","Immunology","Antibody","Gene","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T20:45:49.572961Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}